摘要
Objective:To depict mitochondrial genetic variation for the first time among Anopheles minimus(An.minimus)(Diptera:Culicidae) species from two malaria endemic states of NE India.Methods:Phylogeographic analysis was carried at 9 out of 12 sites oi An.minimus confirmed malaria endemic places.Results:All sequences were Adenine-Thymine rich regions.Transitions were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 ease non synonymous mutation was observed.Three distinct clusters of haplolypes were generated.Haplotvpe diversity and low nucleotide diversity were studied.Overall negative values obtained from Tajima's D test and Fu'sF_S test indicate a recent genetic population expansion.Network analysis has explained sequence diversity that was also shown by mutations in 6 sequences.Conclusions:High genetic diversity observed within the populations of An.minimus species has several possible implications for vector control in the region.
Objective:To depict mitochondrial genetic variation for the first time among Anopheles minimus(An.minimus)(Diptera:Culicidae) species from two malaria endemic states of NE India.Methods:Phylogeographic analysis was carried at 9 out of 12 sites oi An.minimus confirmed malaria endemic places.Results:All sequences were Adenine-Thymine rich regions.Transitions were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 ease non synonymous mutation was observed.Three distinct clusters of haplolypes were generated.Haplotvpe diversity and low nucleotide diversity were studied.Overall negative values obtained from Tajima’s D test and Fu’sF_S test indicate a recent genetic population expansion.Network analysis has explained sequence diversity that was also shown by mutations in 6 sequences.Conclusions:High genetic diversity observed within the populations of An.minimus species has several possible implications for vector control in the region.
基金
provided by the Indian Council of Medical Research(ICMR),Delhi,India
supported by ICMR,Senior Research Fellowship
