Acylated ghrelin and unacylated ghrelin inhibit INS-1 pancreas β-cell injury induced by sustained high glucose

Objective To examine whether ghrelin has beneficial effect on survival of pancreatic INS-1 beta cell.Methods Rat INS-1 cells were cultured separately in 5.6mmol/L glucose(NG group),33.3 mmol/L glucose(HG group),33.3 mmol/L glucose plus 10 nmol/L acylated ghrelin(HG+AG group),and 33.3 mmol/L glucose plus 10μmol/L unacylated ghrelin(HG+UG group).After being incubated for different hours,cell survival rate was determined by MTT.Activity of caspase-3 was estimated by spectrophotometry。