复合芽孢杆菌制剂对肉兔肠道发育和免疫功能的影响

Effects of the Complex Bacillus Preparation on Intestinal Development and Immune Function of Meat Rabbits

【目的】本试验旨在研究不同水平复合芽孢杆菌制剂对肉兔肠道p H、小肠黏膜形态、盲肠菌群和免疫功能的影响。【方法】选取体重相近,35日龄断奶的新西兰肉兔160只,随机分为5组,每组4个重复,每个重复8只(公母各占1/2)。对照组饲喂基础饲粮,抗生素组为在基础饲粮中添加400mg/kg的4%恩拉霉素;试验1、2、3组分别在基础饲粮中添加200mg/kg、300mg/kg和400mg/kg复合芽孢杆菌制剂;试验期8周。【结果】试验1、2、3组均可显著降低肠道p H(P【0.05);试验2、3组显著提高了小肠各段VCR值(P【0.05);试验1、2、3组均显著降低了盲肠中大肠杆菌和总好氧菌数量(P【0.05),同时显著增加乳酸杆菌、双歧杆菌及总厌氧菌数量(P【0.05);试验2、3组显著提高了血清中Ig M、补体C3、C4的含量(P【0.05),试验1、2、3组肠黏膜s Ig A含量均显著高于对照组(P【0.05)。【结论】在饲粮中添加该复合制剂可以促进肠道发育,刺激盲肠有益菌的增殖并抑制有害菌的增殖,同时还可提高机体的免疫功能。本试验条件下,该复合制剂在肉兔饲粮中的适宜添加量为300~400mg/kg。

This study was conducted to investigate the effect of the complex bacillus preparation(CBP) on rab-bit's intestinal p H, small intestinal mucosal morphology, cecal microflora and immune function.160 New Zea-land rabbit of 35 day-old with the same male and female.were random Ly assigned into five groups with 4 rep-licates in each group and 8 rabbits per replicate.Control group fed with basic diet,.Antibiotic group fed withthe basic diet supplemented with enramycin(400mg/kg, 4%),while groups 1, 2, 3 with CBP of 200, 300, 400mg/kg, respectively. The results showed that: compared with the control group, all of group 1, 2 and 3 had asignificanty lower intestinal p H, cecal E. coli and total aerobic bacteria while higher s Ig A of intestinal muco-sal, lactobacilli, bifidobacteria and total anaerobic bacteria(p<0.05). Group 2 and 3 had a significantly smallintestinal VCR and plasma lg M, C3 and C4(p<0.05). It was conducted that CBP can promote intestinal devel-opment, as well as stimulating the proliferation of beneficial bacteria but inhibitting the harmful ones in thececum. Meanwhile, it can also improve the body's immune function. It was suggested that the optimal supple-mentation in meat rabbit's diet was 300~400mg/kg.