钼对鸡免疫功能及抗氧化能力的影响

Effect of molybdenum on immune function and lipid peroxidation in chicken

通过向饮水中添加不同剂量钼,建立试验动物模型:对照组(0 mg/L)、钼Ⅰ组(12.5 mg/L)、钼Ⅱ组(25.0mg/L)、钼Ⅲ组(50mg/L)和钼Ⅳ组(100mg/L)。在试验处理第2周和第5周,给鸡接种新城疫疫苗;在试验处理第7周和第10周,采集血液制备血清,测定血清HI抗体效价和抗氧化指标。结果显示,添加50mg钼显著提高了鸡末期体质量(P<0.05)和平均增质量(P<0.05);钼处理组血清HI抗体效价均高于对照组,其中Ⅲ组和钼Ⅳ组与对照组相比差异极显著(P<0.01);添加不同剂量钼提高了鸡血清TPr和Alb含量,XOD和ACP活性;添加不同剂量钼显著提高了鸡血清SOD、GSH-PX、GSH、NO及T-NOS等酶的活性,且呈剂量依赖关系。结果表明,添加钼能促进鸡生长、提高鸡的免疫功能及抗氧化能力。

The animal model was established by adding different doses of molybdate(Mo)into drinking water as the control group(Mo:0mg/L),Mo Ⅰ group(Mo:12.5mg/L),Mo Ⅱgroup(Mo:25.0mg/L),MoⅢ group(Mo:50.0mg/L)and Mo Ⅳ group(Mo:100.0mg/L).On the2 th week and 5th week,all chickens were immunized by Newcastle disease virus attenuated vaccine.On the 7th week and 10 th week,serum was collected to identify HI antibody titer and antioxidative index.The results showed that the body weight and average weight gain were significantly increased(P<0.05)in adding 50mg/L Mo.Mo Addition significantly increased the content of serum TPr and Alb,and the activity of XOD and ACP in chickens.The activity of SOD,GSH-Px,GSH,NO and T-NOS were also increased significantly by adding Mo in a dose-dependent manner.In conclusion,Mo addition promotes the growth of chicken,significantly increased the immune function and antioxidant ability.