钼镉联合胁迫对山羊组织和器官中微量元素含量的影响

Influence of the content of trace elements in serum,heart,liver,spleen,lung and kidney from goats under combined stresses of the molybdenum and cadmium

36头波尔山羊随机分成4组,选用钼酸铵、氯化镉作为试验钼源、镉源。对照组口服相应剂量去离子水,试验组分为低镉低钼组(Cd 0.5mg/kg+Mo 15mg/kg)、低镉中钼组(Cd 0.5mg/kg+Mo 30mg/kg)、低镉高钼组(Cd0.5mg/kg+Mo 45mg/kg)。试验期为50d,并于第0、25、50天每组3头山羊采血并剖杀取组织样品,测定其钼、镉和铜的含量。结果显示:(1)随着试验攻毒时间的增加,心肌中的钼、镉含量与对照组相比显著升高(P<0.05),且有随灌服钼的增加而升高的趋势。而铜的含量表现为部分显著下降(P<0.05);(2)随着试验攻毒时间和灌服钼质量浓度的增加,肝脏、肾脏、肺脏中的钼、镉含量与对照组相比均显著升高(P<0.05),而铜的含量也显著升高(P<0.05);(3)随着试验攻毒时间和灌服钼浓度的增加,血清中的钼、镉含量与对照组相比均显著升高(P<0.05),而铜的含量表现为部分显著升高(P<0.05);(4)随着试验攻毒时间和灌服钼浓度的增加,脾脏中的钼含量与对照组相比均显著升高(P<0.05),镉、铜含量部分显著上升(P<0.05)。由此可见,羊长期摄入过量钼、镉可使组织器官中钼、镉蓄积量增加,同时限制了机体对铜的利用。

This experiment was conducted to determine influence of the content of trace elements in tissues and organs from goats under combined stresses of the molybdenum and cadmium.36 Boer goats were randomly assigned to four different groups.Select source of molybdenum and cadmium from [(NH4)6Mo7O24·4H2O]and CdCl2,respectively.The control group was treated with the corresponding dose of deionized water.Treatments consisted of low Cd and low Mo group(dose of Cd 0.5mg/kg+Mo 15mg/kg),low Cd and middle Mo group(dose of Cd 0.5mg/kg+Mo 30mg/kg),low Cd and high Mo group(dose of Cd 0.5mg/kg+Mo 45mg/kg).The goats were reared for 50 days under normal condition.Blood samples and tissue samples for groups of three goats were collected on day 0,25 and 50to determine the content of Mo,Cd and Cu from goats.The results are as follows:(1)with the increase of conteracting toxic time,the content of Mo and Cd in cardiac muscle were significantly increased(P<0.05)comparing to the control group,and with the increasing trend of the administrating Mo concentration increased.But the content of Cu were showed partially significantly decreased(P<0.05).(2)With the increase of conteracting toxic time and the perfusion of Mo concentration,the content of Mo,Cd in liver,kidney and lung were significantly increased(P<0.05)comparing to the control group,and the content of Cu were showed significantly increased(P<0.05).(3)With the increase of conteracting toxic time and the perfusion of Mo concentration,the content of Mo,Cd in serum were significantly increased(P<0.05)comparing to the control group,and the content of Cu were showed partially significantly increased(P<0.05).(4)With the increase of conteracting toxic time and the perfusion of Mo concentration,the content of Mo in spleen were significantly increased(P<0.05)comparing to the control group,and the content of Cd,Cu were showed partially significantly increased(P<0.05).The results showed that the content of Mo,Cd in the tissues were increased while the goats took in excessive Mo,Cu long-term,and the use of Cu is limited,which can cause a chronic dearth of Cu in the tissues.