捻转血矛线虫ZJ株滞育虫体的获得及其形态特征

Characterization of Haemonchus contortus ZJ strain diapause

为进一步研究寄生性线虫的滞育机制,参照Adams(1986)的方法,通过瘤胃灌注,将捻转血矛线虫感染性三期幼虫攻入绵羊体内,对绵羊进行处理后,获得捻转血矛线虫滞育虫体。滞育虫体发育程度一致,虫体长(1 067±97)μm(n=18),肠道细胞中含有大量棒状结晶块;性腺开始发育,出现雌驱器原基;颈部出现颈乳突。通过温度检测分析,温度可能不是滞育现象的主要刺激因素。棒状结晶块可以被DAPI特异性着色,其成分可能含有核酸。本试验将为进一步研究捻转血矛线虫的滞育机制及其防治奠定基础。

Diapause larvae of H. contortus ZJ strain were obtained and characterized from abomasums of sheep,which had been injected directly into the rumen with a syringe,according to Adams( 1986),with the aim to investigate the arrested development mechanism. The larvae were characterized by similar length,measuring( 1 067 ± 97) μm( n = 18) and rod-like shape of crystalline inclusion in the intestinal cells. Whole animal DAPI staining revealed genital primordium and the crystalline inclusion in diapause,indicating the crystalline inclusion might contain nucleic acid. The low temperature might not be the key initiated factor inducing diapause through temperature analysis. This study would play roles in researching on the diapause mechanism.