富硒女贞子粗多糖对小鼠免疫功能的影响

Effect of polysaccharides from Se-enriched Ligustrum lucidum fruit on immunologic function in mice

从富硒女贞子及女贞子中提取粗多糖,探讨其对小鼠免疫功能的影响。将90只小鼠随机均分为3组:对照组、富硒女贞子粗多糖组、普通女贞子粗多糖组。对照组灌服生理盐水,试验组小鼠灌服粗多糖液,灌服2次,每次连续5 d。2次灌胃的第3天注射卵清蛋白,二免后测定胸腺脾脏指数;MTT法检测脾淋巴细胞增殖水平;ELISA试剂盒检测血清中IL-2、IL-4、IFN-γ生成水平及相应抗体生成水平。结果显示:与对照组相比,试验组脾脏和胸腺指数明显升高,脾淋巴细胞增殖作用显著提高;IL-2、IL-4、IFN-γ生成水平明显提高,其中IFN-γ与对照组存在显著差异;而对特异性卵清抗体生成影响不大。这表明富硒女贞子粗多糖较普通女贞子粗多糖能更好地促进机体非特异性免疫及细胞免疫,从而增强机体免疫功能,在特异性体液免疫的作用方面则没有表现出明显的规律性,还需进一步研究。

The study was designed to research effects of polysaccharides from Fructus Ligustri Lucidi on immunological function in mice. The mice were randomly divided into three group: Se-enriched Ligustrum lucidum fruit( Se-LLF),Ligustrum lucidum fruit( LLF) and normal control group. Three groups were given Se-LLF and LLF orally for five days. On the third day,ovalbumin was injected to mice as antigens. After the secondary immunization,the level of antibody and the concentration of serum IL-2,IL-4,IFN-γ were determined. The cell proliferation was measured by MTT assay. Index of immune organs were weighed and calculated. The results showed that the levels of interleukin were all higher than those of control group,with no obviouse influnence on the antibodies; also it could promote the growth of mice with immune organs such as spleen and thymus gland and increase the proliferation of mouse spleen cell. Polysaccharides from Se-LLF was better than LLF in enhancing the immune function of mice.