转cry1Ab基因粳稻对稻纵卷叶螟成虫产卵行为的影响

Effects of transgenic cry1Ab japonica rice lines on the oviposition behavior of adult rice leaffolder,Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Pyralidae)

为评价转cry1Ab基因粳稻(KMD1和KMD2)对稻纵卷叶螟Cnaphalocrocis medinalis(Guenée)成虫产卵行为的影响,采用"Y"型嗅觉仪和笼罩以及田间试验等方法对稻纵卷叶螟成虫对转基因水稻的趋性以及其产卵选择性进行了研究,并利用固相微萃取和GC-MS技术测定了这2种Bt水稻及其对照亲本秀水11挥发物的组成及其相对含量。结果表明,稻纵卷叶螟成虫在"Y"型嗅觉仪和小型笼罩内对Bt水稻和对照亲本的选择性差异不显著,而在大型笼罩和田间条件下均显著趋向于在非转基因水稻上产卵,其中大田中稻纵卷叶螟在KMD1、KMD2和对照中的百叶卵量分别为2.20±1.28、1.00±0.77和5.60±2.16粒。水稻挥发物的组成及其相对含量在Bt水稻及其对照亲本之间差异不显著。表明相对于Bt水稻,在田间条件下稻纵卷叶螟成虫趋向于在非转基因水稻产卵,而水稻挥发物可能不是引起这种行为趋性的直接原因。

The widely planted Bacillus thuringiensis( Bt) crops pose risks that target insect pests may evolve resistance to Bt crops. Non-Bt crop refuges play important roles in delaying the resistance development of target insect pests. However,the oviposition behavior of target insect pests may impact establishment of refuges for resistance management of crops transformed with Bt insecticidal protein genes. In the current study,the effects of two transgenic japonica rice lines,KMD1 and KMD2,both expressing Cry1 Ab protein,and their non-transgenic rice line( Xiushui 11) on the oviposition behavior of adult rice leaf folder,Cnaphalocrocis medinalis( Guenée) under laboratory and field conditions. 'Y'-tube olfactometer and cages were used to compare the oviposition behaviors of C. medinalis among different rice types in the laboratory. In addition,the volatiles emitted from Bt and non-Bt rice plants were also compared by using solid phase microextraction( SPME) and linked gas chromatography-mass spectrometry( GS-MS). The results showed that C. medinalis adults did not significantly prefer Bt or non-Bt rice in 'Y '-tube olfactometer and small cages. However,C. medinalis adults significantly preferred non-Bt rice compared with Bt rice in the large cages and under field conditions. The numbers of eggs laid by C. medinalis on KMD1,KMD2,and non-Bt rice plots were 2. 20 ± 1. 28,1. 00 ± 0. 77 and5. 60 ± 2. 16 per 100 leaves,respectively. The components and relative contents of the volatiles emittedfrom Bt and control plants were also similar. C. medinalis adults prefer to lay eggs on non-Bt rice plants compared with Bt rice under field conditions. The differences in the oviposition behavior of C. medinalis adults were unlikely directly caused by changes of the volatiles between Bt and non-Bt rice plants.