高效、准确的玉米粗缩病人工接种鉴定技术

Efficient and accurate artificial inoculation technique of Rice black-streaked dwarf virus to maize

为建立高效、准确的玉米粗缩病人工接种鉴定技术,通过将玉米人工接种水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV),研究了温度对RBSDV在灰飞虱体内循回期的影响以及玉米接种苗龄、接种强度及接种时间对人工接种鉴定效果的影响。结果表明:病毒在灰飞虱体内的循回期随温度升高而缩短,在平均温度分别为18.50、23.50、27.50℃时,循回期分别为36.27、21.76、16.38 d;在玉米芽鞘期至2叶1心期接种,接种强度每苗为7~10头虫,接种时间72~96 h条件下,鉴定效果优于其它处理;感病对照郑单958、掖107、掖478发病充分,病情指数为97.70%~100.00%。用已知抗性水平的玉米自交系和杂交种进行验证,鉴定结果表明该人工接种鉴定技术可客观反映供试玉米材料的实际抗性水平。

In order to develop an effective method for artificial inoculation identification of resistance to Rice black-streaked dwarf virus( RBSDV) in maize materials,four factors including circulative period in the vector Laodelphax striatellus,inoculation stages of the seedlings,inoculation intensity and inoculation time were evaluated in this study. The results revealed that the virus circulative period in the vector decreased as temperature increased. Under the average temperatures of 18. 50,23. 50 and 27. 50 ℃,the circulative periods were 36. 27,21. 76 and 16. 38 d,respectively. When inoculated during coleoptile and2-leaf stages by 7- 10 viruliferous insects per seedling for 72- 96 h,the efficiency was better than other treatments. Under these conditions,inoculated susceptible maize hybrid ‘Zhengdan 958 ' and maize inbred lines of ‘Ye 107 'and ‘Ye 478 'plants showed 97. 70%- 100. 00% RBSDV infection. This technique was verified by inoculating 15 maize inbred lines and 5 hybrids with known resistance levels.The verification results proved that this artificial inoculation identification technique could reflect the RBSDV resistance levels of maize materials.