亚洲玉米螟6-磷酸葡萄糖异构酶基因(Pgi)全序测定及分析

Sequencing and analysis of phosphoglucose-6-isomerase gene( Pgi) of the Asian corn borer,Ostrinia furnacalis( Guenée)

为进一步从核酸水平上研究亚洲玉米螟Pgi基因相关特性,采用反转录PCR及RACE等技术对该基因编码序列及DNA全序列进行了测定,与Gen Bank中其它昆虫Pgi基因相关信息进行比较分析,并构建了系统发育树。结果表明:亚洲玉米螟Pgi基因编码区序列长为1 671 bp,共编码556个氨基酸;其DNA序列全长为10 078 bp(短序列为9 311 bp),由12个外显子与11个内含子镶嵌而成;各外显子长度与鳞翅目大部分昆虫相同,介于95~188 bp之间,内含子序列总长度为8 407 bp(短序列为7 640 bp),各内含子长度介于418~1 547 bp之间,且在内含子3、4、5、11上均发现杂合现象。系统发育结果显示,大部分昆虫Pgi基因c DNA严格按物种聚类,除了双翅目的家蝇Musca domestica与黑森瘿蚊Mayetiola destructor出现一定的交叉现象外,其余没有出现交叉现象。

To further study the relevant features of Pgi gene of Ostrinia furnacalis at the nucleic acid level,the coding sequence and full length of genomic DNA sequence of the gene were detected and analyzed by using PT-PCR,RACE and cloning technology. The sequences were compared with those from other insects,and phylogenetic tree was constructed. The results showed that the Pgi coding region of O. furnacalis was 1 671 bp,coding for 556 aa,and the full length of genomic DNA sequence was 10 078 bp( 9 311 bp for short sequence),which consisted of 12 exons and 11 introns. The length of each exon( 95- 188 bp) was the same as those from most other lepidopteran species. Total length of introns was 8 407 bp( short sequence was 7 640 bp),and the length of each intron was between 418-1 547 bp. The heterozygosity existed in the intron no. 3,4,5 and 11. Phylogenetic analysis showed that the c DNA sequences from most insect Pgi genes were clustered strictly with species, without crossingover,except for Musca domestica and Mayetiola destructor.