我国禾谷缢管蚜微卫星位点扩增稳定性及遗传多样性

Amplifying stability and genetic diversity of microsatellite loci in different Rhopalosiphum padi( Linnaeus) populations in China

为筛选用于我国禾谷缢管蚜种群遗传学研究的微卫星位点,从8个省(市)共9个地区采集282头试虫,采用微卫星PCR产物荧光标记与自动扫描分型方法,研究了8个欧洲禾谷缢管蚜微卫星位点在试虫个体中的扩增稳定性和遗传多样性。结果显示:位点R1.35在9个种群中均不能扩增;位点R5.29b只在7个种群的少数样本中能扩增;位点R2.73、R3.171、R5.10、R5.138、R5.50和R6.3在各种群中均能稳定扩增,这6个位点的无效等位基因频率为0.0044~0.2663,平均等位基因数为2.9~9.3个,观测杂合度为0.047~0.912,其中位点R6.3具有较低的观测杂合度(0.047)和等位基因数(2.9),不适合用于种群遗传多样性研究,而位点R2.73、R3.171、R5.10、R5.138和R5.50均具有较高的杂合度和等位基因数,可用于我国禾谷缢管蚜的种群遗传学研究。

In order to screen polymorphic microsatellite loci which can be used for the population genetics research of Rhopalosiphum padi populations in China,the amplifying stability and genetic diversity of eight previously reported microsatellite loci were analyzed in 282 individuals sampled in nine regions of eight provinces in China.The PCR fragments of the microsatellite loci were labeled fluorescently and scanned automatically.The results showed that the locus R1.35 could not be amplified in any samples of the nine populations,and the locus R5.29b could only be amplified in a few samples of seven populations.Loci R2.73,R3.171,R5.10,R5.138,R5.50 and R6.3 could be amplified stably in samples of all populations.The null allele frequency (Na) of the six stably amplified loci was between 0.0044 and 0.2663;the mean number of allele (NA) ranged from 2.9 to 9.3,and the observed heterozygosity (Ho) was from 0.047 to 0.912.Locus R6.3 showed lower observed heterozygosity (0.047) and mean number of allele (2.9) than other loci,indicating that it could not be used for further population genetics analysis in China.Loci R2.73,R3.171,R5.10,R5.138 and R5.50 showed high observed heterozygosity and mean number of allele,suggesting that they are usable for the genetic diversity and genetic structure research of R.padi populations in China.