仔猪腹泻的大肠杆菌生物膜的筛选和致病力观察

Identification pathogebicity and screening biofilm in Escherichia coli from piglet with diarrhea

采用96孔微量板法,对临床分离鉴定的24株大肠杆菌进行生物膜筛选,得到11株生物膜阳性大肠杆菌,并绘制出4株生物膜强阳性菌株的生长曲线。同时对小鼠进行攻毒试验,结果表明,生物膜阳性菌株的致病力强于生物膜阴性菌株,这为临床预防仔猪腹泻病提供了理论依据。

Diarrhea is one of the major infectious diseases endanger in the lives and health of piglets,The test used 96-well microtiter plate method for the identification and screening biofilm in24 strains E.coli isolated from piglet with diarrhed,11 strains E.coli was positive biofilm,and drawed biofilm growth curve in 4strains of strong positive E.coli.Attacked experiments showed that the pathogebicity in biofilm-positive was more stronger than negative strains.It provided a theoretical basis for prevention of diarrheal disease in piglets.