云南箭竹试管快繁技术研究

Rapid Propagation of Fargesia yunnanensis by Tissue Culture

以云南箭竹成熟种胚在MS(Murashige and Skoog)上诱导的芽作为试验材料,对其试管繁殖进行研究。结果表明:云南箭竹丛生芽的最佳增殖培养基为MS+3 mg·L-1BA(6-苄基腺嘌呤)+1 mg·L-1KT(激动素)+0.001 mg·L-1TDZ(噻苯隆),芽丛生长旺盛,增殖系数达到1.15;最佳生根培养基为1/2 MS+3 mg·L-1IBA(吲哚丁酸),生根率达到100%,根系较长且粗壮;试管苗移栽至泥炭、蛭石、珍珠岩配制比例为1∶1∶1的混合基质中,成活率高达100%。

A tissue culture experiment for Fargesia yunnanensis was conducted by taking the buds induced from mature embryo as explants. The results showed that the optimal medium for bud multiplication was Murashige and Skoog' s( MS) medium supplemented with 3 mg · L- 1BA,1 mg·L- 1KT and 0. 001 mg·L- 1TDZ. Buds grown well with the highest proliferation rate of 1. 15 on the medium. The most suitable rooting medium was 1 /2 MS supplemented with3 mg·L- 1IBA. The rooting rate was up to 100%,and the roots were long and thick. Rooted plantlets were hardened,acclimatized,and transferred to a artificial mixture( peat∶ vermiculite∶ perlite = 1∶ 1∶ 1) with 100% survival.