人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响

Effect of Ginsenoside Rb1Induce Microvascular Vasomotion and the Effect on Sensitive Threshold of Rat Myocardial Microvascular Endothelial Cells

探讨人参皂苷Rb1(GSRb1)对人合谷穴区皮内微血管自律性舒缩活动振幅及大鼠心肌微血管内皮细胞(RMMECs)内游离钙离子瞬时变化的影响。利用激光多普勒血流测定仪结合离子导入仪检测微血管自律性舒缩活动的振幅。利用激光共聚焦显微镜、钙离子荧光探针Fluo-3AM检测ATP引起的RMMECs细胞内钙离子的变化及Rb1对其的影响。结果表明:试验确定了能够引起RMMECs细胞内游离钙离子释放的ATP的最低量为0.5μg·mL-1,瞬时升高差异显著的剂量是0.7μg·mL-1,能够影响RMMECs细胞内钙离子变化的GSRb1的最低量为8μg·mL-1。RMMECs经8μg·mL-1 GSRb1孵育后,能够刺激细胞内游离钙离子释放的ATP的最低量升高为1.5μg·mL-1。本研究说明,提高微血管自律运动振幅的中药成分GSRb1能够通过对RMMECs胞内钙离子的影响,提高RMMECs对ATP的耐受能力(ATP的刺激量由0.5μg·mL-1升高到1.5μg·mL-1)。

The aim of this study was to investigate the effects of Ginsenoside Rb1(GSRb1)induce Hegu acupoint microvascular vasomotion and the effect on sensitive threshold of Rat myocardial microvascular endothelial cells(RMMECs).Microvascular vasomotion was detected by laser doppler blood flow monitor combined with iontophoresis device.The changes of calcium in the RMMECs were measured by laser scanning confocal microscopy combined with calcium fluorescent probe(Fluo-3AM).The results were as follows:The minimum amount of ATP could induce calcium transient increase in RMMECs was 0.5μg·mL-1,the dose of significant difference was0.7μg·mL-1 and the minimum amounts of GSRb1 inducing calcium changes in RMMECs was 8μg·mL-1.After incubation 8μg·mL-1 of GSRb1,the minimum amount of ATP could induce calcium transient increase in RMMECs was 1.5μg·mL-1.Eightμg·mL-1 of GSRb1 can inhibitthe ATP-induced intracellular calcium transient rise,that meant,GSRb1 increased the sensitive threshold of RMMECs to ATP.