维吾尔医学异常黏液质证载体模型大鼠血清代谢组学研究

Metabonomic analysis of the serum from rat model with abnormal balgam syndrome of Uyghur medicine

目的:利用磁共振(magnetic resonance,MR)测定技术探讨异常黏液质证载体模型大鼠血清代谢组分的变化及其与异常黏液质证的关系。方法:将Wistar大鼠(雄性)随机分为正常对照组和异常黏液质证载体模型组,以湿寒饲料(芫荽实和菠菜实)和环境[温度(6±1)℃,相对湿度85%~95%]进行干预,建立异常黏液质证载体模型,并进行鉴定,收集各组大鼠血清,进行磁共振氢谱(1H MR)测定,运用正交偏最小二乘判别(OPLS-DA)分析所采集的图谱,确认差异性代谢组分。结果:与正常组相比,异常黏液质证载体模型大鼠血清中谷氨酸、苯丙氨酸、酪氨酸、柠檬酸、β-羟丁酸、乙酰乙酸、丙酮酸和肌酸等代谢组分含量降低,而α-葡萄糖、β-葡萄糖、乳酸、低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)等代谢组分的含量升高,差异有统计学意义(P<0.05)。结论:异常黏液质证载体模型大鼠三大营养物质分解代谢出现障碍,并处于产能和耗能均较低的状态,三大营养物质及能量代谢障碍可能是异常黏液质证的分子基础。

Objective: To investigate correlation between the change in metabolic components of serum and the abnormal balgam syndrome by using a rat model of abnormal balgam syndrome. Methods: Male Wistar rats were randomly divided into a control group and a test group. According to Uyghur medicine theory, the test group of rats were given wet cold diet(seeds of spinach and parsley, 24 hours) in a cold(6 ℃) and humid(85%-95%, 10 hours) environment for 40 days to establish the rat model of abnormal balgam syndrome. 1H MR based metabonomic analysis of serum was performed. Data was analyzed using Orthogonal Partial Least Squares-Discriminant Analysis(OPLS-DA) software. Results: Compared with the control group, the serum components including glutamate, phenylalanine, tyrosine, citric acid, β-hydrocxy butyrate, acetoacetate, pyruvic acid and creatine were decreased, while the glucose, lactic acid, low density lipoprotein and very low density lipoprotein were increased in the test group(P<0.05). Conclusion: The low energy production and consumption in the rat model of abnormal balgam syndrome suggests that the dysfunctional metabolisms of three major nutrients might be the molecular basis for the abnormal balgam syndrome.