甘草提取物对2,3,7,8-四氯二苯并-p-二噁英致建鲤精密肝切片组织损伤中生化指标及免疫功能的影响

Effects of Glycyrrhiza glabra Extract on Biochemical Indices and Immune Function of Jian Carp(Cyprinus carpiovar. Jian) Precision Cut Liver Slices Injured by 2, 3, 7, 8 tetrachlorodibenzo-p-dioxin

利用精密肝组织切片来评价甘草提取物对2,3,7,8-四氯二苯并-p-二噁英(TCDD)诱导建鲤精密肝切片损伤的保护作用,将制备的肝组织切片分为6个处理组,即Control组、TCDD模型组、甘草提取物对照组、甘草提取物前处理组、甘草提取物后处理组、甘草提取物前后处理组,其中甘草提取物前处理组、甘草提取物后处理组、甘草提取物前后处理组分别换用含0.2、0.4、0.8 mg·m L-1的甘草提取物L-15培养基与精密肝切片共培养,用于检测损伤肝切片培养液中丙氨酸转氨酶(GPT)、天冬氨酸转氨酶(GOT)、超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、免疫球蛋白M(Ig M)的含量变化,结果表明:前处理组甘草提取物可以较好缓解TCDD对肝组织造成的损伤,降低了肝切片培养液中GOT、GPT活性以及MDA、TNF-α、IL-1β、Ig M含量,提高了培养液中SOD活性。这说明甘草提取物可以有效抑制TCDD造成的肝组织损伤,对肝组织具有明显的保护作用。

The hepatoprotective effects of Glycyrrhiza glabra extract(GGE)against TCDD-induced liver injury was evaluated using precision cut liver slices(PCLS). The PCLS were randomly divided into six groups: control group, TCDD model group, GGE group(0.8 mg·m L-1),GGE pre-treatment group, GGE post-treatment group, and GGE pre- and post-treatment group. To PCLS culture system 0.2, 0.4 and 0.8mg·m L-1GGE were added before(pre- treatment), after(post- treatment)and both before and after(pre- and post- treatment)exposure to0.3 μg·L-1TCDD. The culture supernatants of PCLS were collected for detecting activities of glutamic pyruvic transaminase(GPT), glutamic oxaloacetic transaminase(GOT), and superoxide dismutase(SOD), and contents of malondialdehyde(MDA), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β)and immunoglobulin M(Ig M). Results showed that the pre-treatment group had relieving effect on liver injury by TCDD. The GGE significantly inhibited the elevation of GOT, GPT, MDA,TNF-α,IL-1β, and Ig M, but significantly increased level of superoxide dismutase(SOD). Overall results demonstrate the hepatoprotective effects of GGE and support the use of GGE as a hepatoprotec-tive agent in fish.