枯草芽孢杆菌芽孢对猪扁桃体内树突状细胞的影响

Effects of Intranasal Administration with Bacillus subtilis Spores on the Dendritic Cells in Porcine Tonsils

拟研究滴鼻给予猪鼻枯草芽孢杆菌芽孢对咽鼓管扁桃体和软腭扁桃体内树突状细胞数量的影响。10头2月龄杜长大三元杂交仔猪随机分为2组,分别用枯草芽孢杆菌芽孢和PBS进行鼻腔滴鼻,3h后打开鼻腔取出咽鼓管扁桃体和软腭扁桃体。选用MHCII、CD11b和CD16三种猪树突状细胞(DCs)抗体,对咽鼓管扁桃体和软腭扁桃体内DCs进行标记,通过共聚焦显微镜观察滴鼻前后DCs的变化情况。结果表明:应用枯草芽孢杆菌芽孢滴鼻后,咽鼓管扁桃体固有层和淋巴小结内CD11b+CD16+DCs和CD11b+MHCII+DCs的数量显著增加(P<0.05),是正常对照的2~3倍;软腭扁桃体固有层和淋巴小结内CD11b+CD16+DCs和CD11b+MHCII+DCs的数量也显著增加(P<0.05);并且CD11b+MHCII+DCs数量比CD11b+CD16+DCs多,但差异不显著。笔者的研究提示枯草芽孢杆菌芽孢滴鼻能够诱导树突状细胞在扁桃体内聚集,并促进树突状细胞成熟,从而有效提高扁桃体抵抗病原微生物入侵的能力。

Pigs were administrated by intranasal with Bacillus subtilis spores.Three hours later,the numbers of dendritic cells(DC)in tonsils were studied.Ten crossed-bred(Duroc×Landrace×Yorkshine)pigs aged 2months were randomly divided into two groups,Nasal administration with PBS or Bacillus subtilis spores respectively.Three hours later,the tube tonsils and soft palate tonsils were sampled and prepared tissue slices.Three anti-porcine dendritic cells antibodies,MHCII,CD11 band CD16were used to examined the changes of tonsil DC in porcine tonsils.Our results showed that:after intranasal administration of Bacillus subtilis spores,the numbers of CD11b+CD16+DCs and CD11b+MHCII+DCs in tube tonsils have increased significantly(P<0.05),approximately two or three times higher than normal controls;the numbers of CD11b+CD16+DCs and CD11b+MHCII+DCs in soft palate tonsils have increased significantly also(P<0.05).Moreover,the numbers of CD11b+MHCII+DCs were higher than CD11b+CD16+DCs,but the difference was not significant.These results suggested that intranasal administration with Bacillus subtilis spores could induce accumulation of dendritic cells in tonsils and promote dendritic cell maturation,therefore effectively improve the ability of tonsils to resist pathogens invasion.