富硒女贞子对热应激乳腺上皮细胞增殖、凋亡及抗氧化功能的影响

Influence of Se-enriched Ligustrum lucidium Fruit on Proliferation,Apoptosis and Antioxidant Activity of Mammary Epithelial Cells under Heat Stress

为阐明富硒女贞子及其主要成分对高温体外培养乳腺上皮细胞增殖、凋亡及抗氧化功能的影响,以42℃作为热应激温度,37℃作为对照温度,于42℃培养的乳腺上皮细胞分别添加不同质量浓度的富硒女贞子水煎液、齐墩果酸、蛋白质,采用MTT法检测细胞增殖活性,流式细胞仪检测细胞凋亡,黄嘌呤氧化酶法检测SOD活性。结果表明,各质量浓度的富硒女贞子水煎液、齐墩果酸及蛋白质均能显著提高热应激状态下乳腺上皮细胞的增殖活性(P<0.05)、显著降低细胞的凋亡率(P<0.05)、显著提高细胞培养上清中SOD的活性(P<0.05),且富硒女贞子组明显优于普通女贞子组。结果提示,富硒女贞子水煎液、齐墩果酸及蛋白质对热应激引起的乳腺细胞增殖活性降低及细胞凋亡有明显的改善作用,并能提高热应激状态下乳腺细胞的抗氧化功能。

The experiment was conducted to study the effects of se-enriched Ligustrum lucidium Fruit on proliferation,apoptosis and antioxidant activity of mammary epithelial cells cultured under heat circumstance in vitro.The heat stress model temperature was set as 42°C and the temperature 37°C was set as the control.In the experiment,heat-stressed mammary epithelial cells were cultured with different concentration of water extract,protein and oleanolic acid extract of Se-LLF.The growth and apoptosis rate of cultured mammary epithelial cells were detected by MTT and flow cytometry,respectively,and the content of SOD in the supernatant fluid of cultured mammary epithelial cells was detected by xanthine oxidase.The results showed that the proliferation of mammary epithelial cells cultured under high temperature was significantly promoted in each experimental concentration of water extract,protein and oleanolic acid extract of SeLLF(P<0.05).The apoptosis rate decreased significantly(P<0.05),the activity of SOD(P<0.05)was increased significantly,and the results were better than those of the ordinary Ligus-trum lucidum group.In conclusion,water extract,protein and oleanolic acid extract of Se-LLF can not only improve the proliferation and apoptosis rate of heat-stressed mammary epithelial cells,but also improve their antioxidant capacity.