复方中药对肉鸡腹水综合征肺组织NO、ET-1的作用及机制

The Effect and Mechanism of the Compound Recipe of the Traditional Chinese Medicine on NO,ET-1 in Lung of Broiler with Ascites Syndrome

为研究腹水综合征(AS)肉鸡肺组织NO、ET-1及相关指标变化和复方中药对其的防治作用,将316羽14日龄罗斯肉鸡随机分为空白组(N组,常温,常规饲养)、模型组(C组,9~11℃,添加3%猪油和4%鱼粉的高能饲料,0.12%Na+饮水饲养)和复方中药高(T1)、中(T2)、低(T3)剂量组(饲养同C组,每天于饮水中分别给予2、1、0.5mL·kg-1复方中药)以及L-Arg组(L组,饲养同C组,每天于饲料中添加1%L-Arg)。结果表明:与N组相比,35和45日龄C组肉鸡出现典型临床症状,腹水发病率、腹水心脏指数和肺组织NO及ET-1含量、iNOS活性、ETAR mRNA相对表达量显著增加(P<0.01或P<0.05),肺组织cNOS活性和NO/ET-1显著降低(P<0.01);与C组相比,T1、T2和L组均能改善临床症状及上述指标(P<0.01或P<0.05)。肉鸡肺组织NO及ET-1含量、ETAR mRNA相对表达量和iNOS活性升高,cNOS活性降低,导致NO、ET-1失衡,参与了AS的发生;气血双补、行气利水的复方中药能有效防治AS。

The objective of this study was to investigate the changes of the contents of nitric oxide(NO)and endothelin-1(ET-1),and the related items in lung of broilers with ascites syndrome(AS)and the prevention and treatment of the compound recipe of the traditional Chinese medicine on AS.A total of 316 14-day-old Ross broilers were randomly divided into normal group(N,bred with a standard diet at room temperature),control group(C,bred with high-energy diet derived from standard diet added with 3%lard and 4%fish meal and 0.12% Na+in drinking water at 9-11 ℃ to induce AS),compound recipe of the traditional Chinese medicine groups(T1,T2,T3,bred in the same way as group C except a dose of 2,1,0.5mL·kg-1 bw of the traditional Chinese medicine everyday added in drinking water,respectively),and L-arginine group(L,bred in the same way as group C except 1% L-arginine added in diet).The results showed that the broilers in group C presented the typical clinical symptoms of AS at 35 and 45days of age.Compared with group N,the incidence of AS,ascites heart index(AHI),the contents of NO and ET-1,iNOS activity and the relative expression amount of ETAR mRNA in lung of broilers at 35 and 45days of age in group C were increased significantly(P<0.01 or P<0.05),and cNOS activity and the ratio of NO to ET-1were decreased significantly(P<0.01)in group C.The clinical symptoms and items mentioned above were all improved in groups of L,T1 and T2compared with group C(P<0.01 or P<0.05).The study showed that the increases of NO and ET-1content,ETAR mRNA relative expression amount and iNOS activity and the decrease of cNOS activity in lung of broiler induced the unbalance of NO and ET-1which participated in the pathogenesis of AS.The studied compound recipe of the traditional Chinese medicine with the role of tonifying qi-blood and activating qi to excrete water can prevent and treat AS of broiler effectively.