硫化氢上调ABCA1的表达促进HepG2细胞载脂蛋白形成

Hydrogen Sulfide Up-regulates the Expression of ATP-binding Cassette Transporter A1 and Promotes the Formation of Apolipoprotein in HepG2 Cells

目的探讨硫化氢对HepG2细胞ATP结合盒转运体A1(ABCA1)蛋白及载脂蛋白形成的影响。方法将硫化氢供体硫氢化钠(NaHS)以不同浓度(25、50、100、200及400μmol/L)处理HepG2细胞24h后,采用MTT法检测NaHS对HepG2细胞细胞存活率的影响;HepG2细胞以100μmol/LNaHS处理4、12及24h,实时定量PCR检测NaHS对HepG2细胞ABCA1及载脂蛋白A1(ApoA1)、载脂蛋白A2(ApoA2)mRNA的影响,Westernblot检测NaHS对ABCA1、ApoA1及ApoA2蛋白的影响。结果 MTT结果显示,除400μmol/L以外,25、50、100及200μmol/LNaHS对HepG2细胞的生长无抑制作用;Westernblot量效结果显示,NaHS呈浓度依赖性地上调ABCA1蛋白的表达;时效结果显示,100μmol/LNaHS作用4、12及24h,均能上调ABCA1表达及促进培养上清ApoA1、ApoA2的分泌,但不具时间依赖性;实时定量PCR结果显示,ABCA1、ApoA1及ApoA2的mRNA均增加,其变化与蛋白表达变化一致。结论硫化氢可以促进HepG2细胞ABCA1表达,进而促进载脂蛋白形成。

Aim To explore the effects of hydrogen sulfide on the ABCA1 expression and the formation of apolipoprotein. Methods HepG2 cells were treated with different doses of hydrogen sulfide. The cell viability was measured by MTT,the expression of protein was tested by Western blot,the expression of mRNA was detected by real-time PCR. Results Hydrogen sulfide had no influence on cell viability except for 400 μmol / L. At range of 4 to 24 h or0 to 200 μmol / L,hydrogen sulfide could up-regulate the expression of ABCA1 and promote the serection of apolipoproteins in a dose-dependent manner but not in a time-dependent manner,compared with the control group. The mRNA of ABCA1 and apolipoproteins change in accord with the proteins. Conclusion Hydrogen sulfide up-regulates ABCA1 expression and promotes the formation of apolipoproteins.