摘要
目的探讨微小RNA-126(mi R-126)对间质转化中的血管内皮祖细胞(EPC)增殖与迁移的影响。方法分离大鼠骨髓源内皮祖细胞,转化生长因子β1(TGF-β1)诱导EPC的间质转化,慢病毒转染建立mi R-126过表达EPC,通过MMT法检测EPC的增殖,细胞划痕实验与Transwell法检测细胞迁移能力。结果 TGF-β1成功诱导EPC间质转化,mi R-126抑制间质转化中EPC损伤细胞间距的缩短,抑制Transwell小室膜下单位面积的细胞数量。结论 mi R-126过表达抑制间质转化中EPC的增殖与迁移。
Aim To investigate the effect of micro RNA-126( mi R-126) on the proliferation and migration of endothelial progenitor cell( EPC) in the process of endothelial-to-mesenchymal transition( End MT). Methods Rat bone marrow-derived EPC was isolated,and then induced to mesenchymal cells by transforming growth factor-β1( TGF-β1). Overexpression of mi R-126 was constructed in EPC by using lentiviral transfection. The proliferation of EPC was measured by using MTT assay,and migration was detected by using cell injury assay and Transwell assay. Results The transition of EPC from endothelial cells to mesenchymal cells was fully developed after 7 days treatment with TGF-β1( 5 μg/L) mi R-126. Mi R-126 inhibited the decrease of distance between injured EPC in the process of End MT,and attenuated the cells per unit area on the bottom of membrane of Transwell. Conclusion Mi R-126 inhibits the proliferation and migration of endothelial progenitor cells in the process of endothelial-to-mesenchymal transition.
出处
《中国动脉硬化杂志》
CAS
北大核心
2015年第6期556-560,共5页
Chinese Journal of Arteriosclerosis
基金
上海市自然科学基金面上项目(12ZR1417400)
上海市教育委员会科研创新项目(12YZ045)
教育部博士点基金(新教师类)(20110073120096)
关键词
内皮祖细胞
内皮-间质转化
微小RNA-126
增殖
迁移
Endothelial Progenitor Cell
Enthelial-to-mesenchymal Transition
Micro RNA-126
Proliferation
Migration