摘要
目的:研究水飞蓟宾对高脂饮食喂养的C57BL/6J小鼠胰岛β细胞的保护作用及其可能的机制。方法:18只3周龄雄性C57BL/6J小鼠分为普通饮食组(n=6)、高脂饮食组(n=6)及水飞蓟宾干预高脂饮食组(n=6),干预10周后观察各组小鼠空腹血糖、胰岛素、血脂、谷丙转氨酶、肌酐、尿素氮变化及胰岛β细胞的脂质含量、抗氧化水平及凋亡情况,并检测胰腺组织胰岛素诱导基因-1(insulin-induced gene-1,Insig-1)、固醇调节原件结合蛋白-1c(sterol regulatory element binding protein-1c,SREBP-1c)和脂肪酸合成酶(fatty acid synthetase,FAS)的m RNA,以及Insig-1和SREBP-1c蛋白的表达水平。结果:与高脂饮食组相比,水飞蓟宾干预高脂饮食组小鼠胰岛分泌功能明显改善,血糖水平降低(P<0.05),胰岛组织脂质含量、氧化应激水平及凋亡水平均降低(均P<0.05);水飞蓟宾能促进胰岛组织Insig-1的表达,抑制SREBP-1c和FAS的表达(均P<0.05)。三组间谷丙转氨酶、肌酐、尿素氮功能差异无统计学意义(均P>0.05)。结论:水飞蓟宾对高脂饮食小鼠的胰岛β细胞具有保护作用,其机制可能与调节Insig-1/SREBP-1c途径及增加抗氧化活性有关,且长期口服水飞蓟宾安全性良好。
Objective: To explore the eff ect of silibinin on β cells in C57BL/6J mice fed a high-fat diet and the possible mechanisms.Methods: A total of 18 male C57BL/6J mice at 3 weeks old were divided into a normal chow group(n=6), a high-fat diet group(n=6) and a high-fat diet plus silibinin group(n=6). Af ter intervention for 10 weeks, fasting blood glucose(FBG), fasting insulin(FINS), triglycerides(TG), alanine aminotransferase(ALT), creatinine(Cr) and blood urea nitrogen(BUN), lipid metabolism,antioxidant enzyme activities and apoptosis were evaluated. Pancreatic tissues were isolated to examine insulin-induced gene-1(Insig-1), sterol regulatory element binding protein-1c(SREBP-1c) and fatty acid synthetase(FAS) m RNA and protein expression.Results: Compared with the high-fat diet group, the function of insulin secretion was improved, and the level of blood glucose was decreased in the high-fat diet plus silibinin group(P<0.05). The levels of lipid content and oxidative stress and the rates of β cell apoptosis were lower in high-fat diet plus silibinin group than those in the high-fat diet group(both P<0.05). Simultaneously, the silibinin could promote the expression of Insig-1 and depress the expression of SREBP-1c and FAS(all P<0.05). Moreover, there was no significant difference in the levels of serum ALT, Cr and BUN among the 3 groups(all P>0.05).Conclusion: Silibinin can protect β cells of mice fed a high-fat diet, and this effect might be related to, at least partially, increase in its antioxidative ability through regulation of insig-1/SREBP-1c pathway. Moreover, silibinin is safe for long-term treatment.
出处
《中南大学学报(医学版)》
CAS
CSCD
北大核心
2015年第2期165-170,共6页
Journal of Central South University :Medical Science
基金
国家自然科学基金(81200599)
湖南省自然科学基金(13JJ4027)~~