引种巴西甘薯叶抗肿瘤活性部位的筛选

Screening of the anti-tumor active fraction from Ipomoea batatas Lam.(cv.simon) leaves

目的:提取巴西甘薯叶不同极性部位(SM,SM-A,SM-B),进行抗肿瘤活性及剂量相关性研究。方法:以体外培养的人肝癌细胞Hep3B、肺癌细胞A549、胃癌细胞MGC803为研究对象,MTS法分别测定不同极性部位SM,SM-A,SM-B对以上各细胞株增殖活性的影响。结果:SM,SM-A,SM-B对3种肿瘤细胞均有一定的抑制作用;SM-B活性最强,对上述各肿瘤细胞的抑制活性表现为MGC803>A549>Hep3B,其IC50分别为15.17 mg/L(P<0.05),72.64 mg/L(P<0.05)和165.47 mg/L(P<0.05)。结论:巴西甘薯叶具有一定的抗肿瘤活性,SM-B为主要活性部位。

Objective: Three fractions(SM, SM-A, SM-B) were prepared from different polarity parts of Ipomoea batatas Lam.(cv.simon) leaves and the anti-tumor potency as well as the dose-response relations were evaluated.Methods: The anti-tumor activities of fraction SM, SM-A or SM-B were screened by MTS in human hepatic cancer Hep3B cells, lung cancer A549 cells or gastric carcinoma MGC803 cells, respectively.Results: The three fractions all showed anti-tumor activities in three cancer cells with different sensitivity. Among them, SM-B was the most potent fraction with IC50 values at 15.17 mg/L, 72.64 mg/L or 165.47 mg/L in MGC803 cells, A549 cells or Hep3 B cells, respectively(P<0.05).Conclusion: The extraction of Brazil sweet potato leaves displayed anti-tumor activity and SM-B was the most potent fraction.