严重烧伤小鼠肝细胞IFIT1表达与凋亡的关系

Expression of interferon-induced protein with tetratricopetide repeats 1 and liver cell apoptosis in mice with severe burns

目的:探讨严重烧伤急性应激期干扰素诱导含TPR基序的蛋白1(interferon-induced protein with tetratricopetide repeats 1,IFIT1)表达与肝细胞凋亡的关系。方法:健康C57/129小鼠25只,随机分成5组,每组5只:正常对照(0 h)组、烧伤后1,6,12和24 h组。制作烧伤组大鼠20%总体表面积(total body surface area,TBSA)III度烧伤模型。取肝组织,Western印迹观察IFIT1表达;免疫组织化学检测caspase-3和caspase-8表达;脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal deoxynucleotidyl transferase mediated nick end labeling,TUNEL)检测肝细胞凋亡。结果:IFIT1烧伤后1 h开始增高,6 h最高,12 h开始降低,24 h降至最低,组间比较差异有统计学意义(P<0.01);caspase-3和caspase-8烧伤后随时间逐渐增高,组间比较差异有统计学意义(P<0.01);TUNEL法显示烧伤后肝细胞凋亡1 h与0 h比较差异无统计学意义(P>0.05),6 h至24 h呈逐渐增高,组间比较差异有统计学意义(P<0.01)。结论:严重烧伤后IFIT1表达增高可促使肝细胞凋亡的增强。

Objective: To explore the relationship between the expression of interferon-induced protein with tetratricopetide repeats 1(IFIT1) and liver cell apoptosis in the acute stress period after severe burns. Methods: A total of 25 C57/129 adult mice were randomly divided into the normal control group(0 h) and the groups at 1, 6, 12 or 24 after severe burns(n=5 per group). A model with third degree(20% of the total body surface area) burn injury was established and then liver tissues were taken. IFIT1 expression was examined by Western blot. The expression of caspase-3 and-8 was measured by immunohistochemistry. Liver cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated nick end labeling(TUNEL). Results: After burns, IFIT1 expression was increased at 1 h, which reached the highest level at 6 h followed by a decrease at 12 h, which reached minimum level at 24 h. The differences between groups were significant(P<0.01). The caspase-3 and-8 levels significantly increased after burns in a time-dependent manner(P<0.01). Although at 0 h and 1 h there was no significant increase in liver cell apoptosis, the increase reached significance from 6 h to 24 h(P<0.01). Conclusion: The increase in IFIT1 expression after severe burns promotes liver cell apoptosis.