翘嘴鳜(Siniperca chuatsi)转录组EST-SSR位点的信息分析及其多态性检测

ANALYSIS OF EST-SSRs INFORMATION IN SINIPERCA CHUATSI TRANSCRIPTOME AND DETECTION OF POLYMORPHISM

本研究采用高通量测序技术进行了翘嘴鳜(Siniperca chuatsi)转录组学研究,并扩增分析了EST-SSR分子标记的多态性,以期保护翘嘴鳜种质资源及良种选育,发掘其功能性SSR分子标记。结果表明,在翘嘴鳜转录组测序得到的51245条unigenes中共检测出14094个EST-SSR位点,分布于35285条Unigenes中,出现频率为27.51%。翘嘴鳜转录组EST-SSR位点的序列总长度达到195086bp,EST-SSR位点长度分布从10—25个碱基不等,平均长度13bp;翘嘴鳜转录组EST-SSR位点共有90种重复基元;其中,二核苷酸和三核苷酸重复单元是主导类型,分别占总SSR的30.62%和14.23%,且GT/AC、AAG/CCT分别占总SSR的32.12%和6.36%。随机选取100对经济性状相关EST-SSR引物的扩增产物中有24.7%表现为多态性,可作为功能性SSR分子标记开发。

To protect the germplasm resources of fish Siniperca chuatsi, we studied the transcriptome functional SSR molecular makers in high-throughput sequencing technology and detected the polymorphism of EST-SSR molecular makers. Result shows that 14094 EST-SSRs distributed in 35285 unigenes were detected, which is 27.51% of all 51245 unigenes. The total length of S. chuatsi transcriptome EST-SSRs was 195086 bp, the length distribution of transcriptome EST-SSRs was from 10—25bp and the average length was 13 bp. There were 90 types of repeat motifs in the transcriptome EST-SSRs, in which dinucleotide and trinucleotide repeats were two main types for taking 30.62% and 14.23% of all the SSR repeat motifs, respectively. The most abundant di- and tri-motifs were GT/AC and AAG/CCT, occupying 32.12% and 6.36% of all the SSR repeat motifs, respectively. Of 100 EST-SSR microsatellite primers of economic characters that randomly selected, 24.7% were polymorphism primers that could be developed as functional molecular makers of SSR.