紫锥菊提取物对大菱鲆(Scophthalmus maximus)的非特异性免疫功能的影响

EFFECTS OF ECHINACEA PURPUREA EXTRACT ON NONSPECIFIC IMMUNE RESPONSE OF SCOPHTHALMUS MAXIMUS

本文旨在探讨肌肉注射不同浓度的紫锥菊提取物对大菱鲆(Scophthalmus maximus)的非特异性免疫功能的影响,试验选取体重(250±20)g的大菱鲆作为试验动物,分别注射10、20、40mg/m L浓度的紫锥菊提取物,试验共进行28d,每隔7d采集血液测试非特异性免疫指标,第28天用迟缓爱德华氏菌(Edwardsiella tarda)攻毒,连续观察7d,测定其免疫保护率。结果显示肌肉注射紫锥菊提取物对大菱鲆超氧化物歧化酶(SOD)活力和溶菌酶活力(LSZ)的影响极显著(P<0.01);对吞噬细胞吞噬率(PP)的影响显著(0.01<P<0.05),对吞噬细胞呼吸爆发活力的影响极显著(P<0.01)。攻毒试验表明,紫锥菊提取物能够降低迟缓爱德华氏菌攻毒后鱼的死亡率,提高其免疫保护率,尤其是40mg/m L组。本试验研究表明,紫锥菊提取物能显著的提高大菱鲆的非特异性免疫功能,且以注射浓度40mg/m L的效果最好。

Turbot Scophthalmus maximus were treated with herb Echinacea purpurea extract(EE) in different concentrations to investigate the effects of EE on nonspecific immune functions. One-hundred and sixty fish individuals [weight(250±20)g] were divided into 4 groups × 4 replicates × 10 individuals each. The four groups included control group(injected with normal saline) and three treatment groups injected with EE in concentrations of 10, 20, and 40 mg/m L, respectively. The experiment lasted for 28 days, during which blood samples were taken every week for analysis on the activities of superoxide dismutase(SOD), lysozyme(LSZ), phagocyte phagocytosis rate, and respiratory burst activity. At the end of the experiment, fish of treatment groups were challenged with 1×108CFU Edwards iellatarda to investigate the effect of EE on anti-E. tarda infection. The results are listed as follows. Compared to the control, the serum SOD activity and LSZ activity in the 40 and 20 mg/m L groups increased very significantly(P<0.05); the respiratory burst activity in the 40 mg/m L significantly increased(P<0.01); and the phagocyte phagocytosis rate in the 40 mg/m L EE group were increased very significantly(P<0.05). Therefore, EE treatment in a proper concentration, in our case best at 40 mg/m L, could significantly decrease the mortality and enhance nonspecific immunity of turbot against E. tarda.