基质金属蛋白酶-3促进骨肉瘤MG-63细胞的增殖、侵袭及其可能的机制

Matrix metalloproteinase-3 promotes the proliferation and invasion of osteosarcoma MG-63 cells and its possible mechanism

目的:研究基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)对骨肉瘤细胞增殖、侵袭的影响,并初步探讨MMP-3促进骨肉瘤侵袭和转移的机制。方法:收集2013年1月1日至2013年11月1日期间解放军第184医院骨科收治的骨肉瘤患者40例和健康人40名的血液标本,ELISA法检测其中MMP-3含量,并采用SPSS软件分析MMP-3含量与骨肉瘤Enneking分期的相关性。RT-PCR分别检测不同骨肉瘤细胞株U2-OS、HOS-143b、MG-63中MMP-3的表达。MTT法、Transwell侵袭实验观察50、100、200 ng/ml MMP-3刺激或转入MMP-3-shRNA慢病毒(MOI=50、100、200)对MG-63细胞增殖和侵袭的影响,Western blotting方法检测MMP-3过表达或沉默对MG-63细胞中侵袭、转移相关的蛋白p-AKT、核蛋白NF-κB表达的影响。结果:骨肉瘤患者血清中MMP-3的含量高于正常人(F=186.4,P=0.000);且MMP-3含量与骨肉瘤Enneking分期成正相关(r=0.736,P=0.043)。与其他3株细胞相比,MG-63细胞株中MMP-3表达最高(t=5.12,P<0.05)。MMP-3刺激导致MG-63细胞内MMP-3过表达,p-AKT和核内NF-κB表达升高,并促进MG-63细胞的增殖和侵袭;转入MMP-3-shRNA慢病毒导致MMP-3表达沉默,p-AKT和核内NF-κB表达降低,抑制MG-63细胞的增殖和侵袭。结论:MMP-3可能通过增加AKT磷酸化水平和促进NF-κB核转位来促进骨肉瘤细胞的增殖和侵袭。

Objective:To explore the influence and the mechanism of matrix metalloproteinase-3(MMP-3) on invasion and metastasis of osteosarcoma. Methods: 40 cases of patients with osteosarcoma in No. 184 Hospital of PLA from January 1,2013 to November 1,2013 and 40 cases of healthy people were collected,and the serum of the patients with osteosarcoma and the healthy people were collected and the content of MMP-3 was detected with ELISA. And,the correlation between MMP-3 and the pathogenesis of osteosarcoma was also analyzed by SPSS. The mRNA levels of MMP-3 were detected by the method of RT-PCR in different osteosarcoma cell lines such as U2-OS,HOS-143 b,MG-63 cell lines. The proliferation of MG-63 after given different concentration of MMP-3 or knockdown MMP-3 was detected using MTT assay. And the invasion ability was detected by Transwell. Finally,the MMP-3 promotes the invasion and metastasis related proteins of osteosarcoma such as p-AKT,NF-κB were detected by Western blotting. Results: MMP-3 was highly expression in the serum of the patients with osteosarcoma( F = 186. 4,P = 0. 000),and there was a strong positive correlation between MMP-3 exepression and Enneking stage in osteosarcoma( r = 0. 736,P = 0. 043). MMP-3 was highly expression in the cell lines of MG-63( P < 0. 05). The overexpression of MMP-3 stimulated by MMP-3 cultivation increased the expression of p-AKT and the intranuclear NF-κB,then promoted the proliferation and invasion ability of MG-63 cells( P < 0. 05); In contrast,MMP-3- shRNA transfection knockdown the MMP-3,result in reduction of the expression of p-AKT and the intranuclear NF-κB,then suppressed the proliferation and invasion ability of MG-63 cells( P < 0. 05). Conclusion:MMP-3 promotes the invasion and metastasis of osteosarcoma may via increasing the phosphorylation of AKT and promoting the translocation of NF-κB1.