蒺藜苜蓿EMS诱变突变体库的构建及突变体表型的分析

Construction of mutant populations by EMS and phenotypic analysis of Medicago truncatula

突变体是进行功能基因组学研究的重要材料,大量基因已经从各种突变体中得到克隆。本研究利用甲基磺酸乙酯(EMS)诱变蒺藜苜蓿(Medicago truncatula)品种A17种子,鉴定和统计其后代突变型和突变频率,分别获得2 085份M1代和388份M2代突变材料,M2代突变材料中叶色突变性状包括叶斑突变、叶深绿、杂色叶、叶红紫、叶黄萎和白化苗,占群体的5.37%;叶型突变性状包括皱叶、窄叶、卷曲和多叶等,占3.12%;株型突变性状包括高秆、矮化、匍匐、半匍匐、多分枝和少分枝等,占9.11%;开花期突变性状包括早花、晚花等,占1.01%;总的表型突变频率为18.61%。本研究初步构建的蒺藜苜蓿A17 EMS的突变材料一方面创造了蒺藜苜蓿新的种质资源,另一方面可应用于蒺藜苜蓿功能基因组的研究。

Mutants have been used widely to analyze genes function and construct functional genomics study.Many genes were isolated and identified from different mutants of plants.In the present study,the seeds of Medicago truncatula cultivar A17 were treated by EMS and 2 085 of M1 generation and 388 of M2 generation mutants were selected.The mutations with leaf color traits included pigmentation,dark green,variegation,red violet,chloric and albino which count for 5.37% in the mutation populations.The mutations with leaf shape traits included wrinkle,narrow lamina,curly and mixed foliate which count for 3.12% in the mutation populations.The mutations with plant shape traits included taller,dwarf,spreading,sydspor,multi-branch and subramose which count for 9.11%in the mutation populations.The mutations with flowering stage traits included early flower and late flower which count for 1.01%.The total mutant frequency was 18.61% in the M2 population.These mutations provided more germplasm resources for functional genomics study of M.truncatula.