5株根瘤菌接种紫花苜蓿的效果

Inoculation effects of five rhizobial stains to alfalfa

以5株耐盐型苜蓿中华根瘤菌(Sinorhizobium meliloti)(ACCC17537,ACCC17558,ACCC17617,ACCC17631和ACCC17676)为材料,采用包衣接种和液体菌剂拌种两种方式,以不含根瘤菌的包衣处理和不含根瘤菌的液体菌剂拌种处理为对照,在滨海盐渍区盐化潮土上对"中苜1号"紫花苜蓿(Medicago sativa cv.Zhongmu No.1)进行接种试验,筛选出高效菌株及最佳接种方式。结果表明,在盐碱化程度较大、缺乏根瘤菌的土壤中,包衣接种根瘤菌的效果明显优于液体菌剂拌种。另外,5株根瘤菌包衣接种效果差异明显,且苜蓿接种后单株地上部分干重、粗蛋白含量及单株根瘤数均比对照有显著提高(P<0.05)。菌株ACCC17558和ACCC17617接种效果最佳,可使苜蓿单株地上部分干重、单株总根瘤数、单株有效根瘤数和粗蛋白含量分别比对照提高91.4%和164.4%、91.8%和99.6%、99.8%和100.7%、8.6%和12.3%,表明两菌株的接种效果优于其他菌株,是在盐碱土壤中与"中苜1号"紫花苜蓿最佳共生的高效根瘤菌。

Five saline tolerance rhizobial strains(Sinorhizobium meliloti,ACCC17537,ACCC17558,ACCC17617,ACCC17631 and ACCC17676) were inoculated on Medicago sativa cv.Zhongmu No.1 by two inoculation methods in field to screen high efficient rhizobia and inoculation method in coastland.Seed coating and liquid inoculant without rhizobial strains were also included as controls.The results showed that seed coating method was better compared with liquid inoculants if Zhongmu No.1 grew in soil with serious salinization and lack of rhizobia.There were significant difference between the effects of five rhizobial strains on Zhongmu No.1 by seed coating.Dry weight,crude protein,the numbers of total nodules and effective nodules of alfalfa plant inoculating with 5 rhizobial strains significantly increased(P < 0.05) compared with the controls.The effects of ACCC17558 and ACCC17617 on alfalfa were highest.Dry weight,total nodules,effective nodules and crude protein of per alfalfa inoculating with ACCC17558 and ACCC17617 increased 91.4% and 164.4%,91.8% and 99.6%,99.8% and 100.7%,8.6%and 12.3% compared with the control,respectively.The strains of ACCC17558 and ACCC17617 were selected as good inoculants for Zhongmu No.1 alfalfa in saline soil.