地被菊‘紫妍’和‘纽9722’的再生体系建立

Establishment of regeneration system of Chrysanthemum morifolium ‘ziyan'and‘niu 9722'

本研究以地被菊(Chrysanthemum morifolium)两个品种‘紫妍’和‘纽9722’带腋芽的无菌茎段为外植体,在基本培养基中添加不同浓度的6-BA和NAA诱导叶片分化、茎段增殖以及继代生根,进行两个品种的再生体系建立。结果表明,利用2%NaClO对‘紫妍’和‘纽9722’适宜消毒时间分别为6、8min;诱导‘紫妍’叶片不定芽再生的最适培养基是MS+6-BA 2.0mg·L-1+NAA 1.0mg·L-1,愈伤组织诱导率为100%,分化率为92.22%,出芽数为7.60;‘纽9722’叶片不定芽再生的最适培养基是MS+6-BA 2.0mg·L-1+NAA 0.5mg·L-1,愈伤组织诱导率为100%,但分化率仅为45.59%;‘纽9722’茎段增殖的最适培养基是MS+6-BA 2.0 mg·L-1+NAA 0.5mg·L-1,增殖系数高达10.05;‘紫妍’生根的最适培养基是1/2 MS+NAA 0.2 mg·L-1,生根率为100%,生根系数为15.50;‘纽9722’生根的最适培养基为1/2 MS+NAA 0.3mg·L-1,生根率为100%,生根系数为14.87,移栽成活率均为100%。

The regeneration systems of Chrysanthemum morifolium cv.ziyan and C.morifolium cv.niu9722 were established with the aseptic stems with axillary bud as explants and basic medium with different concentrations of 6-BA and NAA.The results showed that the optimal sterilization method was 2% NaClO for 6min for‘ziyan'and 2% NaClO for 8min for‘niu 9722'.The optimal medium for adventitious bud induction from leaves of‘ziyan'was MS+6-BA 2.0mg·L-1+NAA 1.0mg·L-1 with the callus induction ratio of 100%,the adventitious bud differentiation ratio of 92.22%and the reproduction coefficient of 7.60.The optimal medium for adventitious bud induction from leaves of‘niu 9722'was MS+6-BA 2.0mg·L-1+NAA 0.5mg·L-1 with the callus induction ratio of 100%and the adventitious bud differentiation ratio of 45.59%.The optimal medium for stem proliferation of‘niu 9722'was MS+6-BA 2.0mg·L-1+NAA 0.5mg·L-1 with the average multiplication coefficient of 10.05.The optimal medium for rooting of‘ziyan'was 1/2 MS+NAA 0.2 mg·L-1 with the rooting rate of 100% and the average root number of 15.50.The optimal medium for rooting of‘niu 9722'was 1/2 MS+NAA 0.3mg·L-1 with the rooting rate of 100% and the average root number of 14.87.The transferred plants grew well with survival rate of 100%.