麻花秦艽水浸提液对紫花苜蓿和小麦种子萌发和幼苗生长的影响

Effects of aqueous extracts of Gentiana stramine on seed germination and seedling growth of Medicago sativa and Triticum aestivum

用野生麻花秦艽(Gentiana straminea)叶和根的水浸提液对紫花苜蓿(Medicago sativa)和小麦(Triticum aestivum)种子进行处理,探讨麻花秦艽的水浸提液对紫花苜蓿和小麦种子萌发、幼苗生长的影响。结果表明,麻花秦艽叶和根的水浸提液对两种受体植物种子发芽和幼苗生长主要表现为化感抑制作用,且随着浸提液浓度的升高,抑制作用逐渐增强;仅在低浓度0.025 g·m L-1的根水浸提液处理时紫花苜蓿种子萌发和幼苗生长表现一定的促进作用,但当浓度达到0.1 g·m L-1时,紫花苜蓿种子萌发被完全抑制(P<0.05)。麻花秦艽浸提液对紫花苜蓿的抑制作用较小麦强,麻花秦艽叶浸提液的抑制作用较根强。不同浓度麻花秦艽叶和根的水浸提液均能影响紫花苜蓿和小麦幼苗体内抗氧化酶活性,随着浸提液浓度的升高,紫花苜蓿和小麦幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量总体上增加,与对照差异显著(P<0.05)。

Alfalfa( Medicago sativa) and wheat( Triticum aestivum) seeds were treated with different concentrations of aqueous extracts from Gentiana stramine leaves and roots.The results showed that the inhibition effects of aqueous extracts on seed germination and seedling growth of alfalfa and wheat gradually increased with the increase of the G.straminea plant aqueous extract concentration.Only with low concentrations( 0.025 g · m L- 1) G.straminea plant aqueous extracts promoted seed germination and seedling growth of alfalfa.The seeds germination of alfalfa were significantly inhibited( P < 0.05) at a concentration of 0.1 g·m L- 1.The inhibition effects on alfalfa was more serious than that on wheat.Inhibition effects of G.straminea aqueous extracts from leaves was significantly greater than root.The antioxidant enzyme activities of alfalfa and wheat were also affected by aqueous extract.Superoxide dismutase,peroxidase and catalase activities and malondialdehyde content significantly increased( P <0.05) with increasing concentrations.