天然高分子细胞培养基质的研究进展

Advances of Natural Macromolecular Cell Culture Matrix

天然高分子材料如壳聚糖、丝素蛋白和胶原蛋白等具有异体抗原性弱、生物相容性好、可生物降解且能促进细胞粘附、生长和增殖等优点。同人工合成高分子材料相比,天然高分子几乎不含有机溶剂和引发剂等物质,生物安全性高,是细胞培养基质的首选原材料。近年来,随着生物科技的发展,其新功能也不断被发现并受到学术界的广泛重视。本文综述了天然多糖类和天然蛋白质类高分子材料的理化性质及在细胞培养基质领域中的应用,对目前所存在的一些问题进行了描述并预测其发展动向。

Materials in natural polymers have weak allograft antigenicity,good compatibility and biodegradable and can promote cell adhesion,growth and proliferation,Such as chitosan,silk fibroin and collagen.Compare to synthetic polymer,the natural polymers almost do not contain organic solvent and initiators.It showed high biological safety and is preferential to use in cell culture matrix raw materials.In recent years,along with the development of biotechnology,new functions of these natural polymer are being found and valued.In this paper,the physical and chemical properties of natural polysaccharide and protein as well as their application as cell culture matrix are reviewed in details,and the existing problems were described and the development directions of these materials was forecasted.