查尔酮合酶基因对桃果实花色苷代谢的影响

The Effect of Silencing Chalcone Synthase on Anthocyanin Metabolism in Peach

从桃(Prunus persica)果皮中克隆了493 bp的查耳酮合酶(Chalcone Synthase,CHS)基因片段,利用病毒诱导的基因沉默技术(VIGS)构建干扰载体TRV-LIC-CHSi,通过农杆菌渗入法沉默目的基因CHS的表达,从而研究CHS在桃果实花色苷代谢途径中的功能。干扰CHS基因的表达后,CHS的表达量降低88.8%,果皮中花青素糖苷含量下降87%,槲皮素糖苷含量下降35%,绿原酸含量升高57%,原花青素含量、儿茶素含量和山奈酚糖苷含量无显著变化。研究结果表明,沉默CHS基因,会使整个花色苷代谢途径转向绿原酸及其复合物方向。CHS基因调控着类黄酮类物质的代谢方向,是花色苷代谢途径中的重要基因。

In this study,we cloned a 493 bp CHS fragment from the peach(Prunus persica)peal and constructed interference vector TRV-LIC-CHSi using virus-induced gene silencing(VIGS)to study the function of Pp CHS in anthocyanin pathway in peach skin by Agrobacterium infiltration. The interference successfully decreased CHS expression by 88.8% and remarkably modified anthocyanins content in peach skin,specifically,anthocyanin content and quercetin content decreased by 87% and 35% respectively;Chlorogenic acid content increased by 57% up;The content of procyanidin,catechin and kaempferol did not show significant change. Our results demonstrated that the whole anthocyanin pathway was turned to chlorogenic acid and its complexes synthesis when the expression of CHS was severely repressed. CHS is an important gene in anthocyanin synthesis and regulates the relative amount of specific flavonoids.