不同产地中国李资源遗传多样性SSR分析

Analysis of Genetic Diversity of Prunus salicina from Different Producing Areas by SSR Markers

利用均匀分布在8个染色体连锁群上的16对SSR引物对来自3类产区的24份中国李品种的遗传多样性进行分析,结果表明:各引物的多态信息含量(PIC)在0.547~0.783间变化,其中引物CPSCT005最低,引物CPSCT022最高。不同引物间有效等位基因数(Ne)、Nei’s基因多样性(H)和Shannon’s指数(I)分析均存在显著差异,且均为引物CPSCT039最高,引物CPSCT031最低。3类产区所有引物分析表明,Nei’s基因多样性(H)、Shannon’s指数(I)和有效等位基因数(Ne)的关系是:南方品种和北方品种相差不大,均大于国外品种,且南方品种与国外品种先聚到一类。16对SSR引物总共扩出条带86条,其中多态性条带81条,多态性比率为94.19%,平均每对引物扩增位点5.38个。品种聚类分析表明,在遗传距离0.35处,24份中国李材料可分为2大类,大部分北方品种聚为一类,南方品种和国外品种聚为一类,从分子水平支持了以前提出的国外品种起源于中国的说法。

Sixteen pairs SSR primers that evenly distributed on the 8 chromosome linkage group were used to analyze the genetic diversity of 24 Prunus salicina varieties from different producing areas. The result showed that the PIC of each primer was ranging from 0.547 to 0.783,with the lowest primer CPSCT005 and the maximum primer CPSCT022. The effective alleles,Nei's gene diversity and Shannon's information of different primers differed significantly,in which primer CPSCT031 was the lowest while primer CPSCT039 was the maximum. According to the analysis of all primers,the relationship of effective alleles,Nei's gene diversity and Shannon's information of three producing areas were the same:Northern variety and southern variety were less different and greater than foreign variety. The populations of southern variety and foreign variety were clustered together. Sixteen pairs primers expanded 86 sites in total,while the polymorphic loci were 81 with the percentage of 94.19%,and the amplification loci of each pair of primers amplification in average was 5.38. Cluster results showed that in line 0.35,24 species were divided into two groups,and most of the northern varieties gathered for a class whereas the southern varieties and foreign varieties gathered into a group. The results indicated in molecular level that foreign varieties originated from China.