利用Solanum galapagense番茄重组自交系对控制单果质量及果形的QTL定位分析

QTLs Mapping for Tomato Fruit Weight and Fruit Shape in Solanum lycopersicon×S. galapagense Recombinant Inbred Line

利用由野生种番茄(Solanum galapagense)‘LA0317’和普通栽培种番茄(Solanum lycopersicon)‘9706’构建包含130个株系的BC2S8群体,对控制果实质量和果实形状的QTL进行分析,共检测到18个QTL,LOD值介于2.40~5.17之间,可解释8.0%~32.4%的表型变异,其中LOD值大于3的QTL有5个,贡献率超过10%的QTL有8个。与果实质量相关的QTL有5个,加性效应值均为负值。与果形指数相关的QTL有6个,与果实纵径相关的QTL有2个,与果实横径相关的QTL有5个。在分子标记SSR135、In Del_FT-77、TMS66和In Del_FT-143处均聚集了多个控制单果质量和果形的QTL,说明这些位点可能同时对多个不同的果实表型性状起作用,属于多效位点。

The galapagense tomato‘LA0317'was crossed with the cultivated tomato‘9706'to establish a BC2S8 population which includes 130 recombined inbred lines. Eighteen QTLs were detected associated to these fruit morphological traits in total. The LOD values of these QTLs were between 2.40 and 5.17,which can explain 8.0%–32.4% phenotypic variations. Eight QTLs can explain more than 10% variations. Among these QTLs,five QTLs were related to fruit weight and the addictive effects were all negative,six QTLs were related to fruit shape index,two QTLs were related to fruit length and five QTLs related to fruit width. In addition,several QTLs detected controlling different morphological traits were clustered at some molecular makers such as SSR135,In Del_FT-77,TMS66 and In Del_FT-143,which indicated that these pleiotropic loci may simultaneously have impacts on different fruit morphological traits.