堇叶碎米荠硒蛋白的体内抗氧化作用研究

In vivo antioxidation of selenoprotein in Cardamine violifolia

【目的】探讨富硒植物堇叶碎米荠硒蛋白的生理活性,为富硒植物的深度开发利用提供依据。【方法】以从湖北恩施富硒植物堇叶碎米荠叶片中提取的硒蛋白作为硒来源,设置硒质量浓度分别为2.25,4.45,8.90,17.79μg/mL的硒蛋白液灌胃饲喂小鼠4周,研究不同的硒蛋白溶液对小鼠体质量、血硒水平、血液和肝组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量的影响。【结果】硒蛋白液的硒质量浓度为8.90μg/mL时,小鼠体质量增加最多;在本试验条件下,小鼠血硒水平随硒质量浓度的增加而升高;硒质量浓度为4.45~17.79μg/mL的硒蛋白液可明显提高小鼠血液及肝组织的T-AOC、SOD活性和GSH-Px活力,抑制MDA的生成,但硒质量浓度为8.90~17.79μg/mL时,促进效应减缓。【结论】堇叶碎米荠硒蛋白可以促进小鼠的生长及抗氧化能力,最适硒质量浓度为8.90μg/mL。

【Objective】The physiological functions of selenoprotein in hyperaccumulator were investigated to improve the development and utilization of Se-rich plants.【Method】Selenoprotein,purified from leaves of Cardamine violifoliain Enshi,Hubei Province,was feed to Kunming mice by gastro gavage for 4weeks.The mass concentrations of selenium in selenoprotein solution were 2.25,4.45,8.90 and 17.79μg/mL,respectively.Physiological parameters including weight,blood selenium levels,the total antioxidant capacity,SOD,GSH-Px,and MDA in blood and liver tissue were detected.【Result】The weight of Kunming mice increased the most when selenium mass concentration was 8.90μg/mL.Blood selenium levels increased with the increase of selenium concentration.When the mass concentrations of selenium were4.45-17.79μg/mL,selenoprotein significantly enhanced the total antioxidant capacity,SOD,GHS-Px activity and inhibition on MDA formation,but the increasing rates decreased when selenium mass concentrations were 8.90-17.79μg/mL.【Conclusion】Selenoprotein in leaves of Cardamine violifolia promoted the growth and antioxidant ability of Kunming mice,and the optimum selenium mass concentration was8.90μg/mL.