云芝葡聚糖对烟草马铃薯Y病毒的抗性研究

Resistance of Trametes versicolor polysaccharides against potato virus Y

【目的】研究云芝葡聚糖对烟草马铃薯Y病毒(PVY)的抑制作用,为新型杀菌剂云芝葡聚糖水剂的开发应用提供依据。【方法】以PVY枯斑寄主苋色藜(Chenopodium amaranticolor)、系统寄主心叶烟(Nicotiana glutinosa)为材料,采用枯斑法、叶圆片法及室内盆栽试验,测定云芝葡聚糖的抗PVY活性及其诱导抗病性,同时测定其对烟草防御酶POD、PAL活性的影响。【结果】云芝葡聚糖对PVY具有很强的预防作用和诱导抗性,700μg/mL云芝葡聚糖的预防效果达73.98%,诱导抗性达59.84%;对PVY有一定的钝化和抑制增殖作用,其中700μg/mL云芝葡聚糖对PVY的钝化效果为28.79%,对PVY的增殖抑制率达40.25%;云芝葡聚糖处理能提高烟草叶片POD和PAL活性;室内盆栽试验结果表明,云芝葡聚糖对PVY寄主表现出很强的保护活性,保护效果优于治疗效果,接种病毒14d后1.1%云芝葡聚糖水剂300倍液的保护、治疗效果分别为73.33%和38.46%。【结论】云芝葡聚糖是一种很强的免疫增强剂,对PVY的抑制作用显著,提前施用对寄主可起到很好的保护作用。

【Objective】To provide basis for development and application of new fungicide-Trametes versicolor polysaccharides AS,the inhibition of Trametes versicolor polysaccharides against PVY was studied.【Method】Using Chenopodium amaranticolor and Nicotiana glutinosaas materials,the anti-PVY activity of Trametes versicolor polysaccharides was detected by half-leaf method,leaf-discs method,and potted plant method,and the inducible resistance and effects on activity of defensive enzymes POD and PAL were determined.【Result】It was confirmed that polysaccharides had strong preventive effect and induced resistance against PVY,and the preventive effect and induced resistance of 700μg/mL Trametes versicolor polysaccharides were 73.98% and 59.84%,respectively.Polysaccharides also had inactivating and multiplication inhibiting effects against PVY,and the inactivation effect and multiplication inhibition rate of 700μg/mL Trametes versicolor polysaccharides were 28.79% and 40.25%,respectively.Treated by polysac-charides also increased the activity of tobacco POD and PAL.The potted experiment showed that Trametes versicolor polysaccharides had strong protective activity to PVY host,which was better than treatment effect.14 days after inoculating PVY,the protective effect and therapeutic effect of 300 times diluent of polysaccharides were 73.33%,and 38.46%,respectively.【Conclusion】Trametes versicolor polysaccharides were an type of immunopotentiator.They had a strong inhibitory action against PVY and application in advance could achieve better protection effect.