异养硝化-好氧反硝化菌粪产碱杆菌的脱氮特性

Denitriding characteristics of a heterotrophic nitrification-aerobic denitrification strain Alcaligenes faecalis No.4

为了对粪产碱杆菌(Alcaligenes faecalis No.4)的脱氮特性进行研究,提出了不同环境因子对菌株脱氮性能影响的对比实验。结果表明,该菌能利用柠檬酸钠和乙酸钠作为惟一碳源,以柠檬酸钠为碳源时脱氮活性最高,硝酸铵、氯化铵、碳酸铵和硫酸铵4种铵盐均能作为惟一氮源,而硝态氮和亚硝态氮几乎不能被该菌代谢转化;当温度在30~37℃、摇床转速超过120 r/min、p H为6~8之间时,该菌对氨氮的去除效果最好;该菌对高中低浓度的氨氮都具有很好的去除效果,对高浓度的氨氮有很好的耐受性;该菌对0%、3%盐度的适应期较短,对6%的盐度经过较长时间的适应期后,也能够快速地处理氨氮,具有良好的耐盐性。

To research on denitrification characteristics of Alcaligenes faecalis No.4,contrast experiments of the impact of different environmental factors on strain denitrification performance were put forward.The results show that Alcaligenes faecalis No.4 grew and showed nitrogen removal ability when sodium citrate and sodium acetate was supplemented as the sole carbon source respectively,and sodium citrate was the most effective one.Ammonium nitrate,ammonium chloride,ammonium carbonate and ammonium sulfate can be used as the sole nitrogen source respectively,yet nitrate and nitrate nitrogen can hardly be metabolic converted.The most efficient ammonium removal temperature was ranged from 25℃ to 30℃,and the table concentrator speed was more than120 r/min,and the p H was ranged from 6 to 8.The strain shows a good removal effect of different concentrations of ammonia nitrogen,and very good tolerance to high concentrations of ammonia nitrogen as well.The strain has relatively shorter adjustment periods for 0%,3% of the salinity compared to 6%,but it still has good resistance to salinity.