异养硝化-好氧反硝化菌ADN-42的脱氮特性

Nitrogen-removal characteristic of heterotrophic nitrification and aerobic denitrification bacterium ADN-42

从大连海域典型繁茂膜海绵(Hymeniacidon perleve)中筛选出1株耐盐异养硝化-好氧反硝化菌,通过形态观察、生理生化实验和16S rRNA基因序列分析,确定其为假单胞菌属(Pseudomonas),命名为ADN-42。其异养硝化-好氧反硝化条件为氯化铵为氮源,柠檬酸三钠为碳源,温度30℃,C/N值为12,摇床转速150 r/min,NH+4-N初始浓度约300 mg/L,盐度为40 g/L Na Cl,在此条件下菌株84 h时NH+4-N去除率为75.4%,无硝态氮产生,亚硝态氮最大积累量为8.3 mg/L;将菌株投加到NH+4-N和NO-2-N混合体系中,混合系统比仅以NH+4-N为氮源的体系的NH+4-N去除速率提高了12.7%;研究结果表明Pseudomonas sp.ADN-42可能是一株有着良好应用前景的高效耐盐异养硝化-好氧反硝化菌。

A novel heterotrophic ammonium(NH+4-N) removal bacterial strain,designated as ADN-42,was isolated from Hymeniacidon perleve.Strain ADN-42 was identified as Pseudomonas sp.by morphological and16 S rRNA sequence analyses.The ammonia nitrogen removal rate was 75.4% without NO-3accumulation and with a maximum NO-2accumulation of 8.3 mg/L in 84 h under the following conditions:carbon source,sodium citrate;nitrogen source,ammonium chloride;C/N of 12;temperature of 30℃;shaking speed of 150 r/min;p H of 7.0;initial ammonium nitrogen of 300 mg/L;and Na Cl of 40 g/L.When the strain was added to the system containing both NH+4-N and NO-2-N,the NH+4-N removal rate was 12.7% higher than that of the system using only NH+4-N as nitrogen source.The results showed that Pseudomonas sp.ADN-42 could be an efficient heterotrophic nitrification-aerobic denitrification bacterial strain in hypersaline environment.