大气压等离子体射流对水中铜绿微囊藻的灭活作用

Inactivation effect of Microcystis aeruginosa in water by atmospheric-pressure plasma jet

以铜绿微囊藻为研究对象,将空气源大气压等离子体射流(APPJ)引入到液相,构建新型的大气压等离子体射流液相反应体系,考察了不同APPJ处理方式对铜绿微囊藻的灭活效率,观察了放电处理的藻细胞形态,分析了培养基p H的变化和液相产生的主要活性物质(H2O2和O3)对藻灭活的作用,并探讨了APPJ灭活铜绿微囊藻的作用机理。研究结果表明,当电压为7 k V,空气气体流速为4 L/min,藻液吸光度约为0.200,铜绿微囊藻的灭活率达到99.16%;处理后的藻发生细胞变形和破裂;处理后的藻液中产生H2O2和O3等活性物质,同时产生大量的NO-3和NO-2离子,导致液相p H迅速下降。通过考察主要活性物质(H2O2和O3)及藻液p H因素发现,单一因素灭活效果不佳,主要活性物质(H2O2和O3)和p H的组合因素是APPJ灭活水中铜绿微囊藻的主要原因。

Taking Microcystis aeruginosa( M. aeruginosa) as the research object,a novel atmospheric-pressure plasma jet( APPJ) aqueous reaction system was built through air source APPJ introduced into liquid phase.We investigated the effects of the different treatment methods of APPJ on the inactivation efficiency of M. aeruginosa,observed the change of algal cell morphology by discharge treatment,analyzed the effects of main active substances( H2O2,O3) generated in the liquid and p H change on algal inactivation,and discussed the mechanism of M. aeruginosa inactivation. Experimental results showed that the inactivation rate reached 99. 16% under the voltage of 7 k V,the air gas flow rate of 4 L / min and the OD680 of about 0. 200. The treated algal cells were deformed and ruptured,and active substances( H2O2,O3) were produced in algal solution treated by APPJ,meanwhile,large numbers of ions( NO-3,NO-2) were produced,resulting in the rapid decrease of aqueous p H. By the investigation of the factors of main active substances( H2O2,O3) and p H in algal medium,it was found that algal inactivation effect was not better by a single factor,but the combined factors of main active substances( H2O2,O3)and p H were the main reasons for M. aeruginosa inactivation in water by APPJ.