16SrDNA克隆文库解析AO-MBR系统中细菌种群多样性

Analysis of bacterial community composition by 16SrDNA clone library sampling from anoxic-aerobic membrane bioreactor process

采用分子生物学手段16S r DNA克隆文库方法对缺氧/好氧膜生物反应器(AO-MBR)的好氧池与缺氧池中细菌进行了多样性研究。实验结果表明,好氧池污泥样品的克隆文库包括9个类群,其中变形菌(Proteobacteria)和拟杆菌(Bacteroidetes)在文库中所占比例最大,分别为37.04%和14.81%;其次是酸杆菌(Acidobacteria)、未培养菌(uncultured bacterium)、绿菌(Chlorobi)和未培养的绿弯菌(uncultured Chloroflexi bacterium)、浮霉状菌(Planctomycetes),分别为11.11%、11.11%、7.41%、7.41%和5.56%;硝化螺旋菌(Nitrospirae)和裸藻门(Euglenozoa)所占比例相对较小,均为1.85%。缺氧池样品克隆文库包括10个类群,其中变形菌(Proteobacteria)、拟杆菌(Bacteroidetes)和未培养菌(uncultured bacterium)在文库中所占比例最大,分别为27.91%、13.95%和12.79%;其次是浮霉状菌(Planctomycetes)、酸杆菌(Acidobacteria)和绿弯菌(Chloroflexi),在文库中所占比例分别为9.3%、9.3%和9.3%;硝化螺旋菌(Nitrospirae)、裸藻门(Euglenozoa)、芽单胞菌(Gemmatimonadetes)和放线菌(Actinobacteria)所占比例相对较少,分别为6.98%、8.14%、1.16%和1.16%。两池细菌的主要类群相似,但菌属及比例有所差异,变形菌是系统中的主要脱氮菌属。

The bacterial diversity in an anoxic / aerobic membrane bioreactor process( A / O-MBR) was studied by 16 S r DNA cloning and sequencing method. The experimental results showed that the clone library of aerobic sludge sample comprised 9 major groups. The largest fractions were Proteobacteria and Bacteroidetes,which accounted for 37. 04% and 14. 81% of the total clones,respectively. The protions of Acidobacteria,uncultured bacterium,Planctomycetes,Chlorobi and uncultured Chloroflexi bacterium were 11. 11%,11. 11%,5. 56%,7. 41% and 7. 41%,respectively. The relatively lower portions were 1. 85% Nitrospirae and 1. 85%Euglenozoa. The clone library of anoxic sludge sample was found to include 10 major groups,the largest fractions were Proteobacteria,Bacteroidetes and uncultured bacterium,which accounted for 27. 91%,13. 95% and12. 79%,respectively. The protions of Planctomycetes,Acidobacteria and Chloroflexi in this library were 9. 3%,9. 3% and 9. 3%,respectively. The relatively lower portions were 6. 98% Nitrospirae,8. 14% Euglenozoa,1. 16% Gemmatimonadetes and 1. 16% Actinobacteria. The main bacteria groups were similar in the two pools,but the species and proportions were different. Proteobacteria was identified to be the main denitrifer genera in the system.