不同培养条件下锰过氧化物酶(MnP)的合成及其对甲基橙的降解

Manganese peroxidase( Mn P) produced under different culture conditions and for degradation of methyl orange

高效、大规模、低成本合成木质素降解酶是直接采用其降解难降解有机污染物所必须解决的问题。对锰过氧化物酶(Mn P)降解甲基橙和在非灭菌的反应器中连续合成Mn P的可行性进行考察。结果表明,在采用2 mmol H2O2和1.5mmol Mn SO4的降解体系中,获最大脱色效果,且100、200和300 U/L的Mn P可在8 h内将甲基橙分别脱色18%、23%和35%;在非灭菌的反应器水平上实现了固定化培养的P.chrysosporium连续23 d合成Mn P,但Mn P酶活仅为2~23 U/L,难以酶解甲基橙;然而,在摇瓶培养条件下固定化的P.chrysosporium合成的Mn P却能达1 152 U/L。因此,直接采用Mn P对污染物进行降解以及在非灭菌的反应器中持续合成Mn P是可行的,但就在非灭菌条件下如何提高Mn P的合成量还有待开展深入的研究。

The more effective,large-scale and low-cost production of lignin-degrading enzymes is a key step for its application to degrade recalcitrant chemicals. The feasibility of Mn P used to degrade methyl orange and produced in reactor lever under non-sterile conditions was studied. The results showed that maximum decolourization of methyl orange was achieved in the degradation system composed of 2 mmol H2O2 and 1. 5 mmol MnSO4,in which 18%,23% and 35% of methyl orange decolorization reached by using 100 U/L,200 U/L and300 U / L of Mn P in 8 h,respectively. In addition,the immobilized P. chrysosporium achieved a sustained Mn P production(23 days) under non-sterile conditions,but Mn P activity was only 2 ~ 23 U / L,by which it was difficult to degrade methyl orange. However,Mn P activity was up to 1 152 U / L in shake-flask culture system. These results indicated that it was possible for Mn P to degrade methyl orange and to be produced in reactor lever under non-sterile conditions,but further researches are needed to improve the production of Mn P in reactor lever.