添加甘蓝8号染色体片段的大白菜易位系的获得及其遗传稳定性分析

Obtaining and Genetic Stability of Chinese Cabbage – Cabbage Translocation Lines with Fragment of Cabbage Chromosome 8

为创建大白菜—结球甘蓝易位系,以大白菜—结球甘蓝8号单体异附加系(AC8)为材料,对其摘去已开放花朵的花枝进行60Co-γ辐射,然后取新开放花朵花粉授与AC8,获得M1植株,再将M1与AC8亲本大白菜‘85-1’进行回交,对其回交后代进行小孢子离体培养获得DH系。利用286个结球甘蓝相对于大白菜特异的In Del分子标记对DH系植株进行鉴定,结合细胞学观察获得添加甘蓝8号染色体片段的大白菜易位系‘AT8-1’。对易位系‘AT8-1’进行自交,与‘85-1’回交,与大白菜高代自交系‘14-28’和‘14-36’杂交,对其后代进行In Del分子标记鉴定,结果表明该易位系中甘蓝染色体片段不稳定,该片段完整保留率在自交后代群体为61.1%,回交后代群体为26.3%,两个杂交后代群体分别为24.2%与30.0%。后代群体中均未见易位片段变小的植株。

Chinese Cabbage–Cabbage alien addition line AC8 was radiated to obtain M1 plants. Doubled haploid lines were further obtained by microcspores culture for backcross descendants of M1. Two hundred and eighty-six specific In Del molecular markers linked to cabbage linkage group were used for detecting the DH lines. Combining with cytology observation,the translocation line AT8-1 of Chinese cabbage was identified. AT8-1 was selfed,backcrossed and hydridized,then their offsprings were identified by the specific makers. Results showed that the translocation fragment form cabbage was instability,the ratio of keeping entire exogenous fragment in selfing progenies,one backcross progeny and two hybridization progenies were 61.1%,26.3%,24.2% and 30.0%,respectively. In all progeny individuals,none translocation plant was found with smaller fragments than in AT8-1.