6种野生草莓基因组大小估算

Estimation of genome size in six wild strawberry species

【目的】估算野生草苺的基因组大小。【方法】以拟南芥(Arabidopsis thaliana)为内标,利用流式细胞仪测定了6个野生草莓种7个待测样品的基因组大小。【结果】6个野生草莓种的基因组大小分别为:黄毛草莓(274±3.80)Mb,五叶草莓(红果类型)(268±3.73)Mb,五叶草莓(白果类型)(258±5.40)Mb,西南草莓(245±3.28)Mb,东北草莓(241±4.28)Mb,森林草莓(231±3.19)Mb,绿色草莓(230±2.83)Mb。【结论】野生草莓基因组大小的测定为草莓属植物基因组文库的建立及其基因组进化等研究提供了基础数据。

【Objective】The objective of this study was to estimate the genome size of wild strawberry species.【Method】The genome size of six wild strawberry species were measured by flow cytometry,using Arabidopsis thaliana as an internal reference.【Result】The results showed that Cx-values are(274±3.80)Mb for Fragaria nilgerrensis,(268±3.73)Mb for F. pentaphylla(red fruit type),(258±5.40)Mb for F.pentaphylla(white fruit type),(245±3.28)Mb for F. moupinensis,(241±4.28)Mb for F. mandschurica,(231±3.19)Mb for F. vesca and(230±2.83)Mb for F. viridis.【Conclusion】The results of this study provide a basis for future research of genome evolution and the establishment of genomic library in Fragaria.