大熊猫源石膏样小孢子菌的分离鉴定与致病性研究

Identification and pathogenicity of Microsporum gypseum isolated from Ailuropoda melanoleuca

为了研究大熊猫皮肤病的病因及有效防治该病,对患皮肤病的大熊猫病变部位的皮屑进行了真菌学检查与分离,并对分离菌进行了形态学特征鉴定、ITS序列分析以及小鼠的致病性试验。结果分离到的真菌为石膏样小孢子菌(编号为MXP-13-11-1)。分离株MXP-13-11-1的ITS序列的长度为573bp,该片段包括ITS1、5.8SrDNA和ITS2的全部序列以及18SrDNA和28SrDNA的部分序列。BLAST序列比对结果显示,MXP-13-11-1与编号GU348990.1的石膏样小孢子菌的核苷酸序列的相似性为99%。根据形态学鉴定、BLAST比对结果和构建的系统进化树可以将分离株MXP-13-11-1鉴定为石膏样小孢子菌。小鼠致病性试验结果显示,该菌对皮肤致病性强。

In order to study the causes of skin diseases of giant panda and take effective measures to prevention and treatment of them,we collected the pathological scurf of giant panda and conducted the mycological examination and separation.Then the morphological characteristics and ITS sequences of the isolated fungus were identified,and it was subsequently inoculated mice to study its pathogenicity.Results of the isolation and identification demonstrated that the isolated fungus(named as MXP-13-11-1)belonged to Microsporum gypseum.The length of ITS sequences of MXP-13-11-1 was 573 bp,which contained the whole sequences of ITS1,ITS2,and 5.8SrDNA,and partial sequences of 18 SrDNA and 28 SrDNA.BLAST sequence alignment showed that it had 99% similarity with M.gypseum(GU348990.1).Mice infection experiments revealed that it had a strong pathogenicity to skin.