孕期应激对子代雌鼠成年后海马早期生长反应因子-1表达的影响

Effect of prenatal stress on the expression of early growth response-1 of the hippocampus in adult female offsprings in rats

目的:探讨孕期应激对子代雌鼠成年后海马早期生长反应因子-1(Egr-1)表达的影响.方法:SD孕鼠随机分为孕期应激组(PS组)和孕期对照组(对照组).PS组孕鼠在妊娠晚期接受限制性应激,对照组不给予孕期应激.子代成年后,PS组和对照组雌性子代随机又被分为孕期应激-子代对照组(PS-NS)、孕期应激-子代应激组(PS-S)、孕期对照-子代对照组(CON-NS)和孕期对照-子代应激组(CON-S)组.PS-S组和CON-S组先后接受限制性应激和冰水应激.共聚焦免疫荧光化学检测海马CA1、CA3区和齿状回区Egr-1的表达.结果:Egr-1在海马CA1、CA3区和齿状回的表达集中在锥体细胞层,CON-NS组Egr-1的免疫阳性细胞数目较少,与CON-NS组相比,Egr-1的免疫阳性细胞数目在CON-S、PS-NS和PS-S组显著增多,其中以PS-S组Egr-1增多最为明显.结论:孕期应激可上调子代雌鼠成年后海马早期生长反应因子-1的表达,可能参与了孕期应激影响子代成年雌鼠乙醇摄取行为的改变.

Objective:To observe the effect of prenatal stress on the expression of early growth response-1(Egr-1) in the offspring of adult female rat.Methods:Six pregnant SD rats were randomly divided into prenatal stress(PS) group and control group(CON).Rats in the PS guoup were subjected to restraint stress during the late gestational periods,while those in the control were not given prenatal stress.The offsprings of adult female rats were randomly divided into prenatal stressoffspring control group(PS-NS),prenatal stress-offspring stress group(PS-S),prenatal control-offspring control group(CON-NS) and prenatal control-offspring stress group(CON-S).All the rats were offered free choices between water and ethanol solution.Egr-1 was detected by confocal immunofluorescence.Results:Egr-1 was mainly arranged in the hippocampal pyramidale cell layers.The number of Egr-1 positive cells was very small.Compared to CON-NS group,the number of Egr-1 positive cells was significantly increased in CON-S,PS-NS and PS-S groups of hippocampal CA1,CA3 area and dentate gyrus.Conclusion:Prenatal stress can up-regulate the expression of Egr-1,which maybe involved in alcohol preference of adult female rat offspring.