HPLC法测定双黄连片中绿原酸的质量分数

Determination of Chlorogenic acid in Shuanghuanglian tablets by HPLC

探讨高效液相色谱法测定双黄连片中绿原酸含量的方法 ,控制其质量 .Shim -packVP -ODS(4 6mm× 15 0mm ,5 μm)色谱柱 ,流动相为甲醇 -水 -冰醋酸 (2 0∶80∶1) ,流速为 1 0mL/min ,检测波长为 32 7nm .绿原酸在 0 4 0 8～ 2 0 4 0 μg范围内呈良好的线性关系 ,回归方程为A =2 84 0 95 98C +6 894(r=1.0 0 0 ) ,平均回收率为 10 0 .98% (RSD为 1.13% ) ,重复性RSD为 0 .32 % .方法简便、快速、灵敏、准确、重现性好 。

To develop a method for the determination of chlorogenic acid in Shuanghuanglian tables, to control its quality. The Shim-pack VP-ODS column (4.6 mm×150 mm, 5 μm) was used. The mobile phase was consisted of Methanol-water-Acetic acid (20∶80∶1) with the flow rate at 1.0 mL/min and the UV detection wavelength was at 327 nm.The calibration curve was in good linearity over the range of 0.408～2.040 μg,and the regression equation was A=28 409 598 C+6 894(r=1.000). The average recovery was 100.98% (RSD=1.13% n=5); RSD of reproduction was 0.32% (n=5). The method is simple, quick, sensitive, accurate, and reproducible and can be used to control the quality of Shuanghuanglian tablets.