用二维相关红外光谱研究蛋白质分子的热动力学过程

STUDIES ON THERMAL DYNAMICS OF PROTEIN BY TWO-DIMENSIONAL CORRELATION INFRARED SPECTROSCOPY

利用傅立叶变化红外 (FT IR)二维样本和波数相关光谱研究了水溶液中牛血清白蛋白 (Bovineserumalbumin)的热动力学过程 .二维样本相关分析的结果给出了牛血清白蛋白随温度变化的趋势 ,表明牛血清白蛋白剧烈的结构变化开始于 6 6℃ ;而二维波数相关分析提供了在升温过程中牛血清白蛋白结构上变化和各结构变化次序的细节 .

Sample-sample (s-s) and wavenumber-wavenumber (w-w) two-dimensional (2D) correlation analysis of Fourier-transform infrared (FT-IR) spectra were employed to investigate the thermal dynamics of bovine serum albumin (BSA) in the aqueous solution. S-s 2D correlation spectra show the temperature profile of BSA, which indicates that the drastic variations in BSA structure starts at 66 degreesC. However, w-w 2D correlation spectra reveal the variations in the structure of BSA and their orders of variation in the process of temperature elevation. The study demonstrates the key roles of 2D correlation spectra in the analysis of thermal dynamics of protein.