摘要
抗CALLA一次杂交瘤79经8-杂氮鸟嘌呤诱导,成为HAT敏感型细胞系79A,79D;与TNF免疫的脾细胞融合,经筛选鉴定得到了几株分泌抗CALLA和抗TNF双特异性单克隆抗体的三体瘤。然后用羟基磷灰石柱对其中一株E6所分泌的抗体进行了初步纯化,与高压液相法相比,在普通常压色谱条件下取得了较为满意的效果。
Hybridoma cell line 79 secreting anti-CALLA monoclonal antibody, when induced by 8-azaguanine, changed to HAT sensitive cell lines, 79A and 79D. After fusion with spleen cells from BALB/c mice immunized with TNF, several trioma lines secreting bispecific McAbs against TNF and CALLA were selected and identified. Prel-imary purification of E6 cell line products was accomplished by hydroxylapatitc chr-omatography. The findings show that we have achieved relatively satisfactory results by common chromatography as compared with high pressure liquid chromatography.
关键词
白血病
抗原
肿瘤坏死因子
三体瘤
common acute lymphocyte leukemia antigen, CALLA
tumor necrosis factor, TNF
trioma
antibody, monoclonal
bispecificity
