免疫抑制酸性蛋白Ⅱ型在胃癌、贲门癌及癌旁组织中的分布及意义

Expression of Immunosuppressive Acidic Protein-2 in Gastric and Cardiac Carcinoma and Neighbouring Tissues

本文应用抗免疫抑制酸性蛋白Ⅱ型(IAP-2)单克隆抗体-MIL和ABC免疫过氧化物酶法,对17例贲门癌和46例胃癌外科手术切除标本的冰冻切片(27例)和石蜡切片(36例)进行了柒色。结果表明,贲门癌和胃癌的细胞膜及胞浆有IAP-2阳性物质存在,阳性率为90.5％。IAP-2在癌组织中的分布与癌细胞分化有关(P<0.01),分化低的肿瘤细胞阳性率高,着色强。上述结果提示,IAP-2可作为肿瘤标志物之一。可能与肿瘤分化有关,其意义有待进一步研究。

Expression of immunosuppressive acidic protcin-2 (IAP-2) was immunohist- ochemically studied using monoclonal antibody MI2 on 27 fresh frozen and 36 formalin-fixed paraffinc embedded sections of primary tumor lesions obtained from patients with cancer of stomach (46 cases) and cardia (17 cases). IAP-2 was found on the membrane and in the cytoplasm of cancer cells (90.5%). However, abnormal gastric mucosa and neighbouring tissues of tumor were not stained. The staining intensity and positive rate of IAP-2 in cancerous tissues was reversely associated with the degree of differentiation of cancer (p<0.01). It was suggested that IAP-2 may be one of the tumor markers for diagnosis of malignant diseases and may be related to differentiation of tumor.